评估基于16S核糖体DNA的组特异性聚合酶链反应用于检测新鲜及石蜡包埋胃活检标本中的比氏螺杆菌、猫螺杆菌和所罗门螺杆菌。
Evaluation of a group-specific 16S ribosomal DNA-based PCR for detection of Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis in fresh and paraffin-embedded gastric biopsy specimens.
作者信息
De Groote D, Haesebrouck F, van Doorn L J, Vandamme P, Ducatelle R
机构信息
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
出版信息
J Clin Microbiol. 2001 Mar;39(3):1197-9. doi: 10.1128/JCM.39.3.1197-1199.2001.
Abstract
A new specific and sensitive 16S ribosomal DNA-based PCR assay was developed. The assay targets a 78-bp DNA fragment unique to Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis and can be used with freshly frozen and formalin-fixed paraffin-embedded gastric biopsy specimens.
摘要
开发了一种基于16S核糖体DNA的新型特异性灵敏PCR检测方法。该检测方法针对比氏螺杆菌、猫螺杆菌和所罗门螺杆菌特有的78个碱基对的DNA片段，可用于新鲜冷冻和福尔马林固定石蜡包埋的胃活检标本。
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