Zaitlin M
Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.
Philos Trans R Soc Lond B Biol Sci. 1999 Mar 29;354(1383):587-91. doi: 10.1098/rstb.1999.0410.
Proteins unique to tobacco mosaic virus (TMV)-infected plants were detected in the 1970s by electrophoretic analyses of extracts of virus-infected tissues, comparing their proteins to those generated in extracts of uninfected tissues. The genome organization of TMV was deduced principally from studies involving in vitro translation of proteins from the genomic and subgenomic messenger RNAs. The ultimate analysis of the TMV genome came in 1982 when P. Goelet and colleagues sequenced the entire genome. Studies leading to the elucidation of the TMV genome organization are described below.
20世纪70年代,通过对病毒感染组织提取物进行电泳分析,将其蛋白质与未感染组织提取物中产生的蛋白质进行比较,检测到了烟草花叶病毒(TMV)感染植物所特有的蛋白质。TMV的基因组结构主要是通过对基因组和亚基因组信使RNA的蛋白质进行体外翻译的研究推导出来的。1982年,P. 戈莱特及其同事对TMV的整个基因组进行了测序,这才完成了对TMV基因组的最终分析。下面将介绍导致阐明TMV基因组结构的研究。