Hishtil Nurseries, Nehalim 4995000, Israel.
Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Institute, Rishon LeZion 7505101, Israel.
Viruses. 2023 Mar 11;15(3):728. doi: 10.3390/v15030728.
Tomato brown rugose fruit virus (ToBRFV) is a soil-borne virus showing a low percentage of ca. 3% soil-mediated infection when the soil contains root debris from a previous 30-50 day growth cycle of ToBRFV-infected tomato plants. We designed stringent conditions of soil-mediated ToBRFV infection by increasing the length of the pre-growth cycle to 90-120 days, adding a ToBRFV inoculum as well as truncating seedling roots, which increased seedling susceptibility to ToBRFV infection. These rigorous conditions were employed to challenge the efficiency of four innovative root-coating technologies in mitigating soil-mediated ToBRFV infection while avoiding any phytotoxic effect. We tested four different formulations, which were prepared with or without the addition of various virus disinfectants. We found that under conditions of 100% soil-mediated ToBRFV infection of uncoated positive control plants, root-coating with formulations based on methylcellulose (MC), polyvinyl alcohol (PVA), silica Pickering emulsion and super-absorbent polymer (SAP) that were prepared with the disinfectant chlorinated-trisodium phosphate (Cl-TSP) showed low percentages of soil-mediated ToBRFV infection of 0%, 4.3%, 5.5% and 0%, respectively. These formulations had no adverse effect on plant growth parameters when compared to negative control plants grown under non ToBRFV inoculation conditions.
番茄褐色皱果病毒（ToBRFV）是一种土传病毒，当土壤中含有先前感染 ToBRFV 的番茄植物 30-50 天生长周期的根残体时，其土壤介导的感染率约为 3%。我们通过将预生长周期延长至 90-120 天、添加 ToBRFV 接种物以及截断幼苗根系，设计了严格的土壤介导 ToBRFV 感染条件，从而增加了幼苗对 ToBRFV 感染的易感性。这些严格的条件用于挑战四种创新根涂层技术在减轻土壤介导的 ToBRFV 感染的同时避免任何植物毒性影响的效率。我们测试了四种不同的配方，这些配方是在添加或不添加各种病毒消毒剂的情况下制备的。我们发现，在未涂层阳性对照植物 100%土壤介导 ToBRFV 感染的条件下，用含有消毒剂三氯氧化磷（Cl-TSP）的甲基纤维素（MC）、聚乙烯醇（PVA）、硅 Pickering 乳液和高吸水性聚合物（SAP）制备的根涂层配方显示出低比例的土壤介导 ToBRFV 感染，分别为 0%、4.3%、5.5%和 0%。与在非 ToBRFV 接种条件下生长的阴性对照植物相比，这些配方对植物生长参数没有不良影响。
