Homaidan F R, Zhao L, Palaia T, Donovan V, Burakoff R
Department of Physiology, American University of Beirut, New York, New York 10022, USA.
Inflammation. 1999 Apr;23(2):191-205. doi: 10.1023/a:1020249330540.
A rabbit model of TNBS-colitis was used to study the effect of intestinal inflammation on epithelial cell function. Epithelial cells were isolated using a non-enzymatic isolation method without any apparent contamination with infiltrating immune cells. The isolated cells were found to be viable using dye exclusion studies, unidirectional Na+ -fluxes, proliferation assays and morphological studies. The cells, however, showed morphological changes that suggested the presence of increased number of secretory vesicles. This increase correlated well with the increase observed in ion and water secretion as measured by the short-circuit current. Finally, in the colitic tissue the number of PGE2 receptors was greatly reduced with no changes observed in the affinity of PGE2 to its receptor. The reduced number of PGE2 receptors might be due to sensitization of the receptor. In conclusion, we have demonstrated that morphologically and functionally normal epithelial cells can be isolated from the rabbit inflamed distal colon.
采用三硝基苯磺酸（TNBS）诱导的结肠炎兔模型来研究肠道炎症对上皮细胞功能的影响。使用非酶分离方法分离上皮细胞，未发现有浸润免疫细胞的明显污染。通过染料排斥试验、单向Na⁺通量测定、增殖试验和形态学研究发现，分离出的细胞具有活力。然而，这些细胞出现了形态学变化，提示分泌囊泡数量增加。这种增加与通过短路电流测量的离子和水分泌增加密切相关。最后，在结肠炎组织中，PGE₂受体数量大幅减少，而PGE₂与其受体的亲和力未观察到变化。PGE₂受体数量减少可能是由于受体的致敏作用。总之，我们已经证明，可以从兔炎症性远端结肠中分离出形态和功能正常的上皮细胞。
