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菠菜叶片中O-乙酰丝氨酸巯基酶的亚细胞定位

Subcellular localization of O-acetylserine sulfhydrylase in spinach leaves.

作者信息

Fankhauser H, Brunold C, Erismann K H

出版信息

Experientia. 1976 Dec 15;32(12):1494-7. doi: 10.1007/BF01924412.

DOI:10.1007/BF01924412
PMID:1021428
Abstract

A combination of differential centrifugation and isopycnic sucrose density gradient centrifugation of extracts from spinach leaves (Spinacia oleracea L.) shows that about 20% of the O-Acetylserine sulfhydrylase are associated with chloroplasts. No appreciable amounts of O-Acetylserine sulfhydrylase band with mitochondrial and peroxisomal marker enzymes.

摘要

对菠菜叶（菠菜）提取物进行差速离心和等密度蔗糖密度梯度离心相结合的实验表明，约20%的O-乙酰丝氨酸巯基酶与叶绿体相关。没有可检测到的O-乙酰丝氨酸巯基酶条带与线粒体和过氧化物酶体标记酶共分离。

相似文献

1
Subcellular localization of O-acetylserine sulfhydrylase in spinach leaves.菠菜叶片中O-乙酰丝氨酸巯基酶的亚细胞定位
Experientia. 1976 Dec 15;32(12):1494-7. doi: 10.1007/BF01924412.
2
O-acetylserine(thiol)lyase from spinach (Spinacia oleracea L.) leaf: cDNA cloning, characterization, and overexpression in Escherichia coli of the chloroplast isoform.菠菜（Spinacia oleracea L.）叶片中的O-乙酰丝氨酸（硫醇）裂解酶：叶绿体同工型的cDNA克隆、特性分析及在大肠杆菌中的过表达
Arch Biochem Biophys. 1993 Jan;300(1):213-22. doi: 10.1006/abbi.1993.1030.
3
Low-molecular-weight O-acetylserine sulfhydrylase and serine sulfhydrylase of Saccharomyces cerevisiae are the same protein.酿酒酵母的低分子量O-乙酰丝氨酸巯基酶和丝氨酸巯基酶是同一种蛋白质。
J Bacteriol. 1981 Aug;147(2):688-90. doi: 10.1128/jb.147.2.688-690.1981.
4
Purification and characterization of O-acetylserine (thiol) lyase from spinach chloroplasts.菠菜叶绿体中O-乙酰丝氨酸（硫醇）裂解酶的纯化与特性分析
Arch Biochem Biophys. 1992 Jun;295(2):379-90. doi: 10.1016/0003-9861(92)90531-z.
5
Occurrence of low molecular weight O-acetylserine sulfhydrylase in the yeast Saccharomyces cerevisiae.
J Biochem. 1980 Nov;88(5):1419-23. doi: 10.1093/oxfordjournals.jbchem.a133111.
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引用本文的文献

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Localization of adenosine 5'-phosphosulfate sulfotransferase in spinach leaves.腺嘌呤 5'-磷酸硫酸转移酶在菠菜叶中的定位。
Planta. 1978 Jan;143(3):285-9. doi: 10.1007/BF00392000.
2
Intracellular localization of serine acetyltransferase in spinach leaves.丝氨酸乙酰转移酶在菠菜叶中的细胞内定位。
Planta. 1982 Aug;155(4):321-7. doi: 10.1007/BF00429459.
3
Localization of enzymes of assimilatory sulfate reduction in pea roots.豌豆根中同化硫酸盐还原酶的定位。

本文引用的文献

1
Adenosine 5'-sulphatophosphate kinase activity in spinach leaf tissue.菠菜叶组织中的腺苷 5'-硫酸化磷酸激酶活性。
Biochem J. 1973 Jun;134(2):565-79. doi: 10.1042/bj1340565.
2
Studies of sulfate utilization of algae: 15. Enzymes of assimilatory sulfate reduction in euglena and their cellular localization.藻类硫酸盐利用的研究：15. 眼虫同化性硫酸盐还原酶及其细胞定位
Plant Physiol. 1976 Mar;57(3):430-6. doi: 10.1104/pp.57.3.430.
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Isolation of intact plastids from a range of plant tissues.从一系列植物组织中分离完整的质体。
Planta. 1989 Sep;179(2):228-34. doi: 10.1007/BF00393693.
4
Subcellular Distribution of O-Acetylserine(thiol)lyase in Cauliflower (Brassica oleracea L.) Inflorescence.花椰菜（甘蓝型油菜）花中 O-乙酰丝氨酸（硫醇）裂解酶的亚细胞分布。
Plant Physiol. 1992 Mar;98(3):927-35. doi: 10.1104/pp.98.3.927.
5
Sulfate Assimilation in C(4) Plants: Intercellular and Intracellular Location of ATP Sulfurylase, Cysteine Synthase, and Cystathionine beta-Lyase in Maize Leaves.C(4)植物中的硫酸盐同化：玉米叶片中 ATP 硫酸化酶、半胱氨酸合成酶和胱硫醚β-裂合酶的细胞内和细胞间定位。
Plant Physiol. 1984 Jul;75(3):873-5. doi: 10.1104/pp.75.3.873.
6
Regulation of Sulfate Assimilation in Plants : XIII. Assimilatory Sulfate Reduction during Ontogenesis of Primary Leaves of Phaseolus vulgaris L.植物中硫酸盐同化作用的调节： XIII. 菜豆初生叶个体发育过程中的同化性硫酸盐还原作用
Plant Physiol. 1982 Aug;70(2):524-7. doi: 10.1104/pp.70.2.524.
Plant Physiol. 1974 Jun;53(6):870-4. doi: 10.1104/pp.53.6.870.
4
Studies of l-Cysteine Biosynthetic Enzymes in Phaseolus vulgaris L.菜豆 l-半胱氨酸生物合成酶的研究
Plant Physiol. 1972 Oct;50(4):477-9. doi: 10.1104/pp.50.4.477.
5
Localization of Carboxydismutase & Triosephosphate Dehydrogenases in Chloroplasts.羧化歧化酶和磷酸丙糖脱氢酶在叶绿体中的定位
Plant Physiol. 1963 May;38(3):355-60. doi: 10.1104/pp.38.3.355.
6
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
7
Cyanide metabolism in higher plants. IV. Purification and properties of the beta-cyanolanine synthase of blue lupine.高等植物中的氰化物代谢。IV. 蓝羽扇豆β-氰醇腈合酶的纯化及性质
J Biol Chem. 1969 May 25;244(10):2632-40.
8
Enzymatic synthesis of cysteine and S-methylcysteine in plant extracts.植物提取物中半胱氨酸和S-甲基半胱氨酸的酶促合成。
Biochem Biophys Res Commun. 1968 Apr 19;31(2):281-6. doi: 10.1016/0006-291x(68)90743-2.
9
Sulfuration of O-acetylhomoserine and O-acetylserine by two enzyme fractions from spinach.菠菜中两种酶组分对O-乙酰高丝氨酸和O-乙酰丝氨酸的硫化作用。
Biochem Biophys Res Commun. 1968 Apr 19;31(2):275-80. doi: 10.1016/0006-291x(68)90742-0.
10
Purification and characterization of L-serine transacetylase and O-acetyl-L-serine sulfhydrylase from kidney bean seedlings (Phaseolus vulgaris).菜豆幼苗（菜豆）中L-丝氨酸转乙酰酶和O-乙酰-L-丝氨酸巯基酶的纯化与特性分析
Biochim Biophys Acta. 1971 Feb 10;227(2):288-95. doi: 10.1016/0005-2744(71)90061-1.