从一系列植物组织中分离完整的质体。
Isolation of intact plastids from a range of plant tissues.
机构信息
Thimann Laboratories, University of California, Santa Cruz, California 95064.
出版信息
Plant Physiol. 1974 Jun;53(6):870-4. doi: 10.1104/pp.53.6.870.
Abstract
A technique for the isolation of intact plastids from spinach (Spinacia oleracea) and pea (Pisum sativum) leaves, pea roots and castor bean (Ricinus communis) endosperm is described. This technique involves brief centrifugation of whole homogenates on density gradients. Intact plastids were located in the gradient by assaying for triose phosphate isomerase activity. Contamination of the plastic peak with mitochondria and microbodies was estimated by measurement of cytochrome oxidase and catalase, respectively. For three of the four tissues the level of contamination of the plastids by these organelles was 2% or less. The sedimentation behavior of microbodies from different tissues is discussed.
摘要
描述了一种从菠菜（Spinacia oleracea）和豌豆（Pisum sativum）叶片、豌豆根和蓖麻（Ricinus communis）胚乳中分离完整质体的技术。该技术涉及在密度梯度上短暂离心整个匀浆。通过测定磷酸丙糖异构酶活性来确定完整质体在梯度中的位置。通过测量细胞色素氧化酶和过氧化氢酶，分别估计质体峰中与线粒体和过氧化物酶体的污染程度。对于四种组织中的三种，这些细胞器对质体的污染程度为 2%或更低。讨论了来自不同组织的过氧化物酶体的沉降行为。
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