通过体内19F磁共振波谱和色谱法观察替加氟在大鼠肝脏中的代谢。
Metabolism of tegafur in rat liver observed by in vivo 19F magnetic resonance spectroscopy and chromatography.
作者信息
Harada M, Nishitani H, Koga K, Miura I, Umeno Y
机构信息
Department of Radiology, School of Medicine, University of Tokushima.
出版信息
Jpn J Cancer Res. 1992 Apr;83(4):387-91. doi: 10.1111/j.1349-7006.1992.tb00119.x.
Metabolism of tegafur in the rat liver was observed by in-vivo 19F magnetic resonance spectroscopy (MRS). After MRS observation, tegafur and q-fluorouracil (5-FU) in the liver were determined by a chromatographic method for comparison with the results of 19F-MRS. Rats were divided into 3 groups: 1) CCl4-induced liver injury group, 2) uracil combined group, 3) control group. Catabolism to fluoro-beta-alanine was suppressed in both the liver injury group and the uracil combined group. Low peaks of 5-FU and fluoronucleotides could be found only in the uracil combined group. The result of 19F MRS observation of each group was in agreement with the result of determination of tegafur and 5-FU by chromatography. This showed that substances which could be observed by 19F-MRS were in proportion to all intracellular fluoro-containing substances. 19F-MRS can provide direct information on the metabolism of fluoropyimidines non-invasively and it might be a useful aid in choosing suitable chemotherapy for patients.
通过体内19F磁共振波谱法（MRS）观察替加氟在大鼠肝脏中的代谢情况。MRS观察后，采用色谱法测定肝脏中的替加氟和α-氟尿嘧啶（5-FU），以便与19F-MRS的结果进行比较。将大鼠分为3组：1）四氯化碳诱导的肝损伤组，2）尿嘧啶联合组，3）对照组。肝损伤组和尿嘧啶联合组中氟-β-丙氨酸的分解代谢均受到抑制。仅在尿嘧啶联合组中可发现5-FU和氟核苷酸的低峰。每组19F MRS观察结果与色谱法测定替加氟和5-FU的结果一致。这表明19F-MRS可观察到的物质与所有细胞内含氟物质成比例。19F-MRS能够无创地提供关于氟嘧啶代谢的直接信息，可能有助于为患者选择合适的化疗方案。
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