McMenamin P G
Department of Anatomy and Human Biology, University of Western Australia, Nedlands, Perth 6907, Western Australia.
Br J Ophthalmol. 1999 May;83(5):598-604. doi: 10.1136/bjo.83.5.598.
BACKGROUND/AIMS: Dendritic cells (DC) and macrophages are components of the immune cell populations in the uveal tract whose density, distribution, turnover, and function may play a role in the maintenance of immunological homeostasis in the eye. Little is known of these cells in the mouse eye despite this being the predominant experimental model in many studies of ocular immune responses and immunoinflammatory mediated eye diseases. The aim of the present study was to obtain further immunophenotypic data on resident tissue macrophages and DC populations in the mouse uveal tract.
Pieces of iris, ciliary body, and choroid dissected from perfusion fixed BALB/c mice were incubated whole in a variety of anti-macrophage and DC monoclonal antibodies (mAbs). Labelled cells were visualised using either single or double immunoperoxidase techniques.
Quantitative analysis and double immunolabelling revealed that 80% of F4/80(+) cells (a mAb that recognises both DC and macrophages) in the iris are macrophages (SER4(+)). The iris contained a network of Ia+ cells (412 (SD 130) cells/mm2) of which two thirds appear to be DC. A similar pattern was observed in the ciliary body and choroid. Only a few DC in the uveal tract were very weakly reactive for mAbs which recognise B7-1 (CD80), B7-2 (CD86), beta2 integrin (mAb N418), and multivesicular bodies associated with antigen presentation (mAb M342).
The present study reveals that the mouse uveal tract, like the rat, contains rich networks of DC and resident tissue macrophages. The networks of resident tissue macrophages in the mouse uveal tract closely resemble similar networks in non-ocular tissues. The phenotype of uveal tract DC suggests they are in the "immature" phase of their life cycle, similar to Langerhans cells of the skin, thus implying their role in situ within the eye is antigen capture and not antigen presentation.
背景/目的：树突状细胞（DC）和巨噬细胞是葡萄膜中免疫细胞群体的组成部分，其密度、分布、更新和功能可能在维持眼部免疫稳态中发挥作用。尽管在许多眼部免疫反应和免疫炎症介导的眼病研究中，小鼠眼是主要的实验模型，但对这些细胞在小鼠眼中的情况知之甚少。本研究的目的是获得关于小鼠葡萄膜中驻留组织巨噬细胞和DC群体的进一步免疫表型数据。
从经灌注固定的BALB/c小鼠中分离出的虹膜、睫状体和脉络膜组织块，整体与多种抗巨噬细胞和DC单克隆抗体（mAb）一起孵育。使用单免疫过氧化物酶技术或双免疫过氧化物酶技术对标记细胞进行可视化。
定量分析和双免疫标记显示，虹膜中80%的F4/80(+)细胞（一种识别DC和巨噬细胞的mAb）是巨噬细胞（SER4(+)）。虹膜含有Ia+细胞网络（412（标准差130）个细胞/mm2），其中三分之二似乎是DC。在睫状体和脉络膜中观察到类似的模式。葡萄膜中只有少数DC对识别B7-1（CD80）、B7-2（CD86）、β2整合素（mAb N418）和与抗原呈递相关的多囊泡体（mAb M342）的mAb反应非常弱。
本研究表明，小鼠葡萄膜与大鼠一样，含有丰富的DC和驻留组织巨噬细胞网络。小鼠葡萄膜中驻留组织巨噬细胞网络与非眼部组织中的类似网络非常相似。葡萄膜DC的表型表明它们处于生命周期的“不成熟”阶段，类似于皮肤中的朗格汉斯细胞，因此意味着它们在眼内的原位作用是抗原捕获而非抗原呈递。
