Olsson J M, Xia L, Eriksson L C, Björnstedt M
Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institutet, Huddinge University Hospital, Sweden.
FEBS Lett. 1999 Apr 1;448(1):190-2. doi: 10.1016/s0014-5793(99)00363-4.
Ubiquinol is an endogenously synthesized lipid-soluble antioxidant. Regeneration of ubiquinol from the oxidized form is essential to the maintenance of its antioxidant function. We demonstrated that lipoamide dehydrogenase can reduce ubiquinone to ubiquinol. Zinc increased the rate of the NADPH-dependent reduction more than 10-fold. The concentration ubiquinone resulting in the half-maximal rate of reduction was approximately 5 microM in the presence and 4 microM in the absence of zinc. These data may explain how ubiquinone is reduced to the active antioxidant ubiquinol, which plays such an important role in protecting against oxidative stress and lipid peroxidation.
泛醇是一种内源性合成的脂溶性抗氧化剂。泛醇从氧化形式的再生对于维持其抗氧化功能至关重要。我们证明硫辛酰胺脱氢酶可将泛醌还原为泛醇。锌使NADPH依赖性还原速率提高了10倍以上。在有锌存在时，导致还原半最大速率的泛醌浓度约为5 microM，在无锌时为4 microM。这些数据可能解释了泛醌如何还原为活性抗氧化剂泛醇，泛醇在抵御氧化应激和脂质过氧化中发挥着如此重要的作用。
