HprK对HPr和Crh的磷酸化作用，是枯草芽孢杆菌果聚糖酶操纵子分解代谢物阻遏信号通路的早期步骤。
Phosphorylation of HPr and Crh by HprK, early steps in the catabolite repression signalling pathway for the Bacillus subtilis levanase operon.
作者信息
Martin-Verstraete I, Deutscher J, Galinier A
机构信息
Unité de Biochimie Microbienne, CNRS URA 1300, Institut Pasteur, F-75724 Paris, France.
出版信息
J Bacteriol. 1999 May;181(9):2966-9. doi: 10.1128/JB.181.9.2966-2969.1999.
Abstract
Carbon catabolite repression (CCR) of Bacillus subtilis catabolic genes is mediated by CcpA and in part by P-Ser-HPr. For certain operons, Crh, an HPr-like protein, is also implicated in CCR. In this study we demonstrated that in ptsH1 crh1 and hprK mutants, expression of the lev operon was completely relieved from CCR and that both P-Ser-HPr and P-Ser-Crh stimulated the binding of CcpA to the cre sequence of the lev operon.
摘要
枯草芽孢杆菌分解代谢基因的碳分解代谢物阻遏（CCR）由CcpA介导，部分由磷酸化丝氨酸-HPr介导。对于某些操纵子，类HPr蛋白Crh也参与CCR。在本研究中，我们证明在ptsH1 crh1和hprK突变体中，lev操纵子的表达完全解除了CCR的抑制，并且磷酸化丝氨酸-HPr和磷酸化丝氨酸-Crh都刺激了CcpA与lev操纵子cre序列的结合。
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