Yoshida H, Yoshida T, Iizuka T, Sakakura T, Fujita S
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kyoto University, Japan.
Oral Dis. 1999 Jan;5(1):50-4. doi: 10.1111/j.1601-0825.1999.tb00064.x.
The aim of this investigation was to study the immunohistochemical localization of MMP-3 and tenascin in the temporomandibular joint and to compare it with control specimens.
Localizations of matrix metalloproteinase-3 (MMP-3) and tenascin in the temporomandibular joint disc and the synovial membrane in 26 human temporomandibular joint samples (internal derangement of TMJ; n = 16, and control; n = 10) by an immunohistological method with monoclonal antibodies specific to human MMP-3 and tenascin.
MMP-3 was not distributed in control specimens while it was observed in the internal derangement cases. MMP-3 showed two staining profiles: (1) diffuse staining was observed within the stroma of severely deformed disc with osteophyte and/or disc displacement (three of 16 specimens); and (2) the localization was specifically detected on the surface of severely hypertrophic synovial membrane (six of 16 specimens). The latter localization pattern resembled that of the tenascin on the surface of the severely hypertrophic synovial membrane.
Comparative localization of MMP-3 and tenascin revealed intense staining for both in the synovial membrane presenting inflammation, proliferation and hypertrophy. These findings suggest that tissue repair and remodeling in the temporomandibular joint disc and the inflammatory hypertrophic reaction in the synovial membrane might proceed at the same time.
本研究旨在探讨基质金属蛋白酶-3（MMP-3）和腱生蛋白在颞下颌关节中的免疫组化定位，并与对照标本进行比较。
采用针对人MMP-3和腱生蛋白的单克隆抗体免疫组化方法，对26例人类颞下颌关节样本（颞下颌关节内紊乱；n = 16，对照；n = 10）的颞下颌关节盘和滑膜中MMP-3和腱生蛋白进行定位。
MMP-3在对照标本中未分布，而在颞下颌关节内紊乱病例中观察到。MMP-3呈现两种染色模式：（1）在伴有骨赘和/或盘移位的严重变形盘的基质内观察到弥漫性染色（16例标本中的3例）；（2）在严重肥厚的滑膜表面特异性检测到定位（16例标本中的6例）。后一种定位模式类似于腱生蛋白在严重肥厚滑膜表面的定位模式。
MMP-3和腱生蛋白的比较定位显示，在出现炎症、增殖和肥厚的滑膜中两者均有强烈染色。这些发现表明，颞下颌关节盘的组织修复和重塑以及滑膜中的炎性肥厚反应可能同时进行。
