Prajna N V, Sathish S, Rajalakshmi P C, George C
Aravind Eye Hospital, Madurai, India.
Indian J Ophthalmol. 1998 Dec;46(4):229-32.
Anterior chamber aspirate cultures were done for 66 patients who underwent either an uncomplicated intracapsular cataract extraction, extracapsular cataract extraction with posterior-chamber intraocular lens implantation, or phacoemulsification with posterior-chamber intraocular lens implantation. The aspirate was obtained at the time of wound closure. The aspirates were immediately transferred to the microbiology laboratory where one drop of the aspirate was placed on a glass slide for gram stain, and the remainder was unequally divided and inoculated into blood agar, chocolate agar and thioglycolate broth. The cultures were incubated at 37 degrees C with 5% CO2 and held for 5 days. Of 66 patients 4 (6%), had smear-positive anterior chamber aspirates. None of the aspirates showed any growth on any of the 3 culture media used. None of the eyes in the study developed endophthalmitis. This study concludes that there is no contamination of the anterior chamber by viable bacteria after cataract surgery, irrespective of the mode of intervention.
对66例接受单纯囊内白内障摘除术、后房型人工晶状体植入的囊外白内障摘除术或后房型人工晶状体植入的超声乳化白内障吸除术的患者进行了前房穿刺液培养。穿刺液在伤口闭合时获取。穿刺液立即被送往微生物实验室，其中一滴被置于载玻片上进行革兰氏染色，其余部分不等分后接种到血琼脂、巧克力琼脂和硫乙醇酸盐肉汤中。培养物在37℃、5%二氧化碳条件下孵育5天。66例患者中有4例（6%）前房穿刺液涂片阳性。在所使用的3种培养基上，所有穿刺液均未显示任何生长。该研究中的眼睛均未发生眼内炎。本研究得出结论，白内障手术后前房不会被活菌污染，无论采用何种干预方式。
