Kurata Shinya, Ohtsuki Takashi, Wada Takeshi, Kirino Yohei, Takai Kazuyuki, Saigo Kazuhiko, Watanabe Kimitsuna, Suzuki Tsutomu
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Bldg. FSB-301, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture, 277-8562, Japan.
Nucleic Acids Res Suppl. 2003(3):245-6. doi: 10.1093/nass/3.1.245.
Post-transcriptional modification at the first (wobble) position of the tRNA anticodon participates in precise decoding of the genetic code. We recently identified a novel taurine-containing modified uridine (tau m5U; 5-taurinomethyluridine) at the wobble position of mammalian mitochondrial tRNAs and found lack of this modification in mutant mitochondrial tRNAs from human pathogenic cells of the mitochondrial encephalomyopathies, investigate molecular pathogenesis of the diseases, decoding activity of wobble uridines with or without C5 modification was measured using E. coli cell-free translation system. It has been revealed that C5 modification has a functional role for stabilizing U:G wobble base pair.
转运RNA（tRNA）反密码子第一位（摆动位）的转录后修饰参与遗传密码的精确解码。我们最近在哺乳动物线粒体tRNA的摆动位鉴定出一种新型的含牛磺酸的修饰尿苷（tau m5U；5-牛磺甲基尿苷），并发现来自线粒体脑肌病患者致病细胞的突变线粒体tRNA中缺乏这种修饰。为研究这些疾病的分子发病机制，我们使用大肠杆菌无细胞翻译系统测量了具有或不具有C5修饰的摆动尿苷的解码活性。结果表明，C5修饰对于稳定U:G摆动碱基对具有功能性作用。
