Zuo Y, Riley D E, Krieger J N
Department of Urology, School of Medicine, University of Washington, Seattle 98195, USA.
J Parasitol. 1999 Apr;85(2):203-7.
Trichomonas vaginalis is a flagellated protozoan, a representative of 1 of the earliest known eukaryotic lineages. Trichomonas vaginalis lacks centrioles but possesses basal bodies. We report here the cell cycle-dependent flagellar dynamics of T. vaginalis. By immunofluorescence, we found that T. vaginalis flagella duplicated during S-phase, segregated toward the nuclear poles, and then emanated from the spindle poles at mitosis. This behavior strongly parallels that of centrioles and other spindle pole-associated structures variously termed centrosomes, spindle pole bodies, or microtubule organizing centers. These observations support the hypothesis that flagellar forces may have provided motile forces for spindle pole alignment in an ancestral eukaryote.
阴道毛滴虫是一种有鞭毛的原生动物，是已知最早的真核生物谱系之一的代表。阴道毛滴虫缺乏中心粒，但具有基体。我们在此报告阴道毛滴虫细胞周期依赖性的鞭毛动力学。通过免疫荧光，我们发现阴道毛滴虫的鞭毛在S期复制，向核极分离，然后在有丝分裂时从纺锤极发出。这种行为与中心粒以及其他各种被称为中心体、纺锤极体或微管组织中心的纺锤极相关结构的行为非常相似。这些观察结果支持了这样一种假说，即鞭毛力可能为祖先真核生物中纺锤极的排列提供了运动力。
