Noël C, Gerbod D, Delgado-Viscogliosi P, Fast N M, Younes A Ben, Chose O, Roseto A, Capron M, Viscogliosi E
Institut Pasteur, Unité Mixte INSERM-IPL U547, 1 Rue du Professeur Calmette, B.P. 245, 59019, Lille cedex, France.
Parasitol Res. 2003 Apr;89(6):487-94. doi: 10.1007/s00436-002-0811-4. Epub 2003 Jan 21.
The behavior of microtubular structures during division was followed by immunofluorescence in Trichomonas vaginalis using an anti-alpha-tubulin monoclonal antibody together with nuclear staining by DAPI, allowing us to describe successive mitotic stages. In contrast to recent reports, we showed that: (1) the microtubular axostyle-pelta complex depolymerized during division, (2) the flagella were assembled during mitosis, and (3) the flagellar number was restored in each daughter kinetid before cytokinesis. Observation of griseofulvin-treated T. vaginalis cells revealed that the elongation of the mitotic spindle or paradesmosis was not the main motile force separating the daughter kinetids to opposite poles during division, suggesting the existence of other mechanisms and/or molecules involved in this morphogenetic event. Examination of treated cells re-incubated in fresh medium showed the nucleation of microtubules radiating from the perinuclear area, the origin of which is discussed. Finally, we confirm the effectiveness of griseofulvin against T. vaginalis and propose that this antifungal drug could be a promising antitrichomonal agent.
利用抗α-微管蛋白单克隆抗体通过免疫荧光法追踪阴道毛滴虫分裂过程中微管结构的行为，并结合用4',6-二脒基-2-苯基吲哚（DAPI）进行核染色，从而使我们能够描述连续的有丝分裂阶段。与最近的报道相反，我们发现：（1）微管轴柱-盾状体复合体在分裂过程中解聚；（2）鞭毛在有丝分裂期间组装；（3）在胞质分裂之前，每个子动质体中的鞭毛数量得以恢复。对经灰黄霉素处理的阴道毛滴虫细胞的观察表明，有丝分裂纺锤体或连丝的延长并非分裂期间将子动质体分离到相对两极的主要动力，这表明存在其他参与这一形态发生事件的机制和/或分子。对在新鲜培养基中重新孵育的处理过的细胞进行检查，发现微管从核周区域放射状成核，本文对其起源进行了讨论。最后，我们证实了灰黄霉素对阴道毛滴虫的有效性，并提出这种抗真菌药物可能是一种有前景的抗滴虫剂。
