Juliano C, Rubino S, Zicconi D, Cappuccinelli P
J Protozool. 1986 Feb;33(1):56-9. doi: 10.1111/j.1550-7408.1986.tb05557.x.
The flagellated protozoon Trichomonas vaginalis, parasite of the human urogenital tract, possesses a well developed microtubule system organized in highly differentiated structures. We have shown by immunoblotting that monospecific anti-sheep brain tubulin antibodies are able to react with the microtubular tubulin of T. vaginalis. These antibodies were used to study the microtubular system of T. vaginalis both in interphase and mitosis by indirect immunofluorescence. The interphase microtubular pattern, characterized by an axostyle, a pelta, four anterior flagella, and a recurrent flagellum, displayed remarkable changes at the onset of mitosis: the axostyle disappeared, and two pole bodies connected by a short spindle became evident; chromosomal fibers arose while pole-to-pole fibers elongated. The last phases of mitosis were marked by the disappearance of chromosomal fibers, the appearance of two small axostyles, and the depolymerization of the pole-to-pole bundle. At the end of mitosis, the normal interphase microtubule pattern was observed.
阴道毛滴虫是人类泌尿生殖道的寄生虫，属于有鞭毛的原生动物，其微管系统发育良好，由高度分化的结构组成。我们通过免疫印迹法证明，单特异性抗羊脑微管蛋白抗体能够与阴道毛滴虫的微管微管蛋白发生反应。这些抗体被用于通过间接免疫荧光研究阴道毛滴虫在间期和有丝分裂期的微管系统。间期微管模式以轴柱、盾板、四条前鞭毛和一条后鞭毛为特征，在有丝分裂开始时显示出显著变化：轴柱消失，由短纺锤体连接的两个极体变得明显；染色体纤维出现，极间纤维伸长。有丝分裂的最后阶段以染色体纤维的消失、两个小轴柱的出现以及极间束的解聚为标志。在有丝分裂结束时，观察到正常的间期微管模式。
