人肠道细菌LJ-22株介导甘草酸转化为18β-甘草次酸-3-O-β-D-葡萄糖醛酸苷
Biotransformation of glycyrrhizin to 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide by Streptococcus LJ-22, a human intestinal bacterium.
作者信息
Kim D H, Lee S W, Han M J
机构信息
College of Pharmacy, Kyung-Hee University, Seoul, Korea.
出版信息
Biol Pharm Bull. 1999 Mar;22(3):320-2. doi: 10.1248/bpb.22.320.
By human intestinal bacteria, glycyrrhizin (18beta-glycyrrhetinic acid-3-O-[beta-D-glucuronopyranosyl-(1-->2)-betaD-glucuro nopyranoside], GL) was metabolized to 18beta-glycyrrhetinic acid (GA): the main pathway metabolized GL to GA by glucuronidases of Bacteroides J-37 and Eubacterium sp. GLH and the minor pathway metabolized GL to 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide (GAMG) by beta-glucuronidase of Streptococcus LJ-22. Beta-Glucuronidase from Streptococcus LJ-22 hydrolyzed GL to GAMG (not GA). The molecular weight and optimal pH of the enzyme were 240 kDa and 5-6.
通过人体肠道细菌，甘草酸（18β-甘草次酸-3-O-[β-D-吡喃葡萄糖醛酸基-(1→2)-β-D-吡喃葡萄糖醛酸苷]，GL）被代谢为18β-甘草次酸（GA）：主要途径是由拟杆菌J-37和真杆菌属GLH的葡萄糖醛酸酶将GL代谢为GA，次要途径是由链球菌LJ-22的β-葡萄糖醛酸酶将GL代谢为18β-甘草次酸-3-O-β-D-葡萄糖醛酸苷（GAMG）。来自链球菌LJ-22的β-葡萄糖醛酸酶将GL水解为GAMG（而非GA）。该酶的分子量和最适pH分别为240 kDa和5 - 6。
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