Raskin D M, de Boer P A
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106-4960, USA.
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4971-6. doi: 10.1073/pnas.96.9.4971.
Accurate placement of the division septum at the midpoint of Escherichia coli cells requires the combined action of a general division inhibitor (MinC), a site-specific suppressor of division inhibition (MinE), and an ATPase (MinD) that is required for proper functioning of both MinC and MinE. We previously showed that a functional MinE-Gfp fusion accumulates in a ring structure at/near the middle of cells. Here we show that functional Gfp-MinD accumulates alternately in either one of the cell halves in what appears to be a rapidly oscillating membrane association-dissociation cycle imposed by MinE. The results indicate that MinD represents a novel type of dynamic cellular element in bacteria, with multiple roles in directing the division apparatus to the middle of the cell.
隔膜准确地定位在大肠杆菌细胞的中点需要一种通用分裂抑制剂（MinC）、一种分裂抑制的位点特异性抑制因子（MinE）以及一种ATP酶（MinD）的共同作用，MinD是MinC和MinE正常发挥功能所必需的。我们之前表明，功能性的MinE-Gfp融合蛋白在细胞中部/附近以环状结构积累。在此我们表明，功能性的Gfp-MinD在细胞的两个半部分之一中交替积累，这似乎是由MinE施加的快速振荡的膜结合-解离循环。结果表明，MinD代表了细菌中一种新型的动态细胞元件，在将分裂装置引导至细胞中部方面具有多种作用。
