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大肠杆菌中振荡Min系统的生长依赖性浓度梯度。

Growth-dependent concentration gradient of the oscillating Min system in Escherichia coli.

作者信息

Parada Claudia Morais, Yan Ching-Cher Sanders, Hung Cheng-Yu, Tu I-Ping, Hsu Chao-Ping, Shih Yu-Ling

机构信息

Institute of Biological Chemistry, Academia Sinica , Taipei, Taiwan.

Institute of Chemistry, Academia Sinica , Taipei, Taiwan.

出版信息

J Cell Biol. 2025 Feb 3;224(2). doi: 10.1083/jcb.202406107. Epub 2024 Dec 2.

DOI:10.1083/jcb.202406107
PMID:39621132
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11613459/
Abstract

Cell division in Escherichia coli is intricately regulated by the MinD and MinE proteins, which form oscillatory waves between cell poles. These waves manifest as concentration gradients that reduce MinC inhibition at the cell center, thereby influencing division site placement. This study explores the plasticity of the MinD gradients resulting from the interdependent interplay between molecular interactions and diffusion in the system. Through live cell imaging, we observed that as cells elongate, the gradient steepens, the midcell concentration decreases, and the oscillation period stabilizes. A one-dimensional model investigates kinetic rate constants representing various molecular interactions, effectively recapitulating our experimental findings. The model reveals the nonlinear dynamics of the system and a dynamic equilibrium among these constants, which underlie variable concentration gradients in growing cells. This study enhances quantitative understanding of MinD oscillations within the cellular environment. Furthermore, it emphasizes the fundamental role of concentration gradients in cellular processes.

摘要

大肠杆菌中的细胞分裂受到MinD和MinE蛋白的复杂调控，它们在细胞两极之间形成振荡波。这些波表现为浓度梯度，可降低细胞中心的MinC抑制作用，从而影响分裂位点的定位。本研究探讨了系统中分子相互作用和扩散之间相互依存的相互作用所导致的MinD梯度的可塑性。通过活细胞成像，我们观察到随着细胞伸长，梯度变陡，细胞中部浓度降低，振荡周期稳定。一个一维模型研究了代表各种分子相互作用的动力学速率常数，有效地概括了我们的实验结果。该模型揭示了系统的非线性动力学以及这些常数之间的动态平衡，这是生长细胞中可变浓度梯度的基础。这项研究增强了对细胞环境中MinD振荡的定量理解。此外，它强调了浓度梯度在细胞过程中的基本作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecc7/11613459/3add5fdc0197/jcb_202406107_figs7.jpg
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Probing transient excited states of the bacterial cell division regulator MinE by relaxation dispersion NMR spectroscopy.
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Active Transport of Membrane Components by Self-Organization of the Min Proteins.膜成分的主动运输通过 Min 蛋白的自组织。
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