Jenssen H L, Werner H, Köhler H
Acta Biol Med Ger. 1976;35(11):1499-503.
Supernatants of blood lymphocytes from immunized guinea pigs after incubation with purified protein derivative (PPD) were fractionated on Sephadex G-75. The fractions were tested for activity to change the electrophoretic mobility of macrophages. Dependent on incubation time, three different regions of activity with an average molecular weight of 13 000, 40 000 and more than 100 000 were estimated.
用纯化蛋白衍生物(PPD)孵育免疫豚鼠的血液淋巴细胞上清液,然后在葡聚糖凝胶G - 75上进行分级分离。检测各组分改变巨噬细胞电泳迁移率的活性。根据孵育时间,估计出三个不同的活性区域,其平均分子量分别为13000、40000和超过100000。