Jahnke L S
Department of Plant Biology, University of New Hampshire, Durham, New Hampshire 03824, USA.
Anal Biochem. 1999 May 1;269(2):273-7. doi: 10.1006/abio.1999.4052.
A liquid chromatographic (HPLC) method has been developed for direct quantitative determination of methane sulfinic acid (MSA) produced by hydroxyl radical oxidation of dimethyl sulfoxide. This method measures MSA directly by HPLC separation and electrochemical oxidation following rapid extraction from intact cells. MSA can be measured in tissue extracts at 0.04 nmol (equivalent to 2 microM). Using this technique, MSA production in paraquat-treated bean leaves is demonstrated. When compared with the widely used dye-binding technique, this method simplifies the preparation of the extract by eliminating two steps required in the dye-binding method: removal of interfering lipophilic compounds and the derivitization (color reaction) of the MSA.
