[Determination of raffinose type sugars in seeds of leguminous plants by high pressure liquid chromatography (HPLC)].

A method was developed for the determination of galacto-sugars: raffinose, stachiose and verbascose, in bean and broad bean seeds by high-pressure liquid chromatography. The effect of various concentrations of the ethanolic extraction solvent and of other solutions (methanol and acetonitrile) on the effectiveness of sugar extraction from bean was investigated. The optimal composition of the mobile phase was established. It was found that the effectiveness of raffinose and stachiose extraction from bean seeds was highest when 40-60% ethanol was the extraction solvent, and when the proportion of bean to the extraction solvent by weight was 2:25. The presented HPLC technique, as compared with gas chromatography, allows for threefold shortening of the duration of one chromatographic analysis.