Czygier M, Mroczko B, Szmitkowski M, Jankowski D
Zakład Diagnostyki Biochemicznej Akademii Medycznej w Białymstoku.
Med Dosw Mikrobiol. 1998;50(3-4):151-60.
GM-CSF is a hematopoietic growth factor. In vitro it stimulates the proliferation of myeloid progenitors and formation of granulocyte and macrophage colonies. It was found that GM-CSF in vitro is also stimulated the function of mature granulocytes, but we have no information about such influence in vivo. The purpose of this investigation was the evaluation in vivo of the GM-CSF effect on phagocytosis, bactericidal activity, and lysosome enzyme activities in granulocytes. GM-CSF was injected into mice subcutaneously during 5 consecutive days in the dose of 1 microgram/kg/d. The examination of the percent of cell phagocytizing bacteria (Staphylococcus aureus), NBT test, bactericidal activity and activation of acid phosphatase, alkaline phosphatase, peroxidase and esterase was performed every day and an evident increase of the tested parameters was found. These results prove in vivo activation of granulocytes by GM-CSF.
粒细胞-巨噬细胞集落刺激因子是一种造血生长因子。在体外，它刺激髓系祖细胞的增殖以及粒细胞和巨噬细胞集落的形成。研究发现，粒细胞-巨噬细胞集落刺激因子在体外还能刺激成熟粒细胞的功能，但我们尚无其在体内影响的相关信息。本研究的目的是在体内评估粒细胞-巨噬细胞集落刺激因子对粒细胞吞噬作用、杀菌活性和溶酶体酶活性的影响。连续5天以1微克/千克/天的剂量皮下注射粒细胞-巨噬细胞集落刺激因子至小鼠体内。每天进行吞噬细菌（金黄色葡萄球菌）的细胞百分比检查、硝基四氮唑蓝试验、杀菌活性以及酸性磷酸酶、碱性磷酸酶、过氧化物酶和酯酶的活性检测，结果发现所检测参数明显增加。这些结果证明粒细胞-巨噬细胞集落刺激因子在体内可激活粒细胞。
