Czygier M, Mroczko B, Olszańska D, Dakowicz L, Szmitkowski M, Kemona H
Zakład Diagnostyki Biochemicznej Akademii Medycznej w Białymstoku.
Med Dosw Mikrobiol. 1998;50(3-4):141-9.
Interleukin-3 is a multipotential hematopoietic growth factor, which like other colony stimulating factors (CSFs) is effective "in vitro" stimulation of the mature cells function. It was found that IL-3 synergistically with GM-CSF and G-CSF stimulated the proliferation of the granulocytes. Therefore the purpose of this investigation was the evaluation "in vivo" of the influence of IL-3 on the phagocytosis, bactericidal activity, and enzyme activities of granulocytes. IL-3 was injected into mice subcutaneously during 5 days in dose 1 microgram/kg/d. The examination of the percent of cells phagocytizing bacteria (Staphylococcus aureus), NBT test and bactericidal activity, were performed every day and evident increase of the tested parameters was found. Additionally the enzyme activities in primary granules were measured and showed on increase of acid phosphatase and peroxidase activity.
白细胞介素-3是一种多潜能造血生长因子，它与其他集落刺激因子（CSF）一样，能在“体外”有效刺激成熟细胞的功能。研究发现，白细胞介素-3与粒细胞巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）协同刺激粒细胞的增殖。因此，本研究的目的是在“体内”评估白细胞介素-3对粒细胞吞噬作用、杀菌活性和酶活性的影响。以1微克/千克/天的剂量，在5天内对小鼠进行皮下注射白细胞介素-3。每天对吞噬细菌（金黄色葡萄球菌）的细胞百分比、硝基四氮唑蓝（NBT）试验和杀菌活性进行检测，发现检测参数明显增加。此外，还测量了初级颗粒中的酶活性，结果显示酸性磷酸酶和过氧化物酶活性增加。
