Filippovich I I, Nozdrina V N, Kupchinenko V V, Oparin A I
Biokhimiia. 1976 Apr;41(4):708-17.
Membrane-bound ribosomes of chloroplasts, isolated from pea seedlings during grana formation, can be partially liberated by 0.5 M KCl and 0.001 M puromycin. In case of mature chloroplasts, after the completion of grana formation process these agents are inefficient, and liberation of ribosomes and polyribosomes may be achieved only after solubilization of thylakoid membranes by 1% Triton X-100. Electron microscopic study of the heavy membrane fraction of young chloroplasts reveals electron-transparent membranes, containing rings and discs of thylakoids with a diameter of about 2 mum. These rings are liberated together with ribosomes under the action of 0.5 M KCl; Triton X-100 liberates equally-sized annular polyribosomes. The rings detected in chloroplast membranes at early stages of development are regarded as structures, precursor grana thylakoids, and the annular polyribosomes included into them as immediate participants of thylakoid morphogenesis.
从豌豆幼苗中分离出的处于基粒形成阶段的叶绿体膜结合核糖体，可被0.5M氯化钾和0.001M嘌呤霉素部分释放。对于成熟叶绿体，在基粒形成过程完成后，这些试剂效果不佳，核糖体和多核糖体的释放只有在类囊体膜被1% Triton X-100溶解后才能实现。对幼龄叶绿体重膜部分的电子显微镜研究显示，电子透明膜中含有直径约2μm的类囊体环和盘。这些环在0.5M氯化钾的作用下与核糖体一起释放；Triton X-100释放出大小相同的环状多核糖体。在叶绿体发育早期阶段在叶绿体膜中检测到的环被视为结构，即前体基粒类囊体，其中包含的环状多核糖体被视为类囊体形态发生的直接参与者。
