van Muyden R C, ter Harmsel B W, Smedts F M, Hermans J, Kuijpers J C, Raikhlin N T, Petrov S, Lebedev A, Ramaekers F C, Trimbos J B, Kleter B, Quint W G
Department of Gynecology, Reinier de Graaf Gasthuis, Delft, The Netherlands.
Cancer. 1999 May 1;85(9):2011-6.
The correlation between human papillomavirus (HPV) infection and tumor prognosis in 159 Russian women with cervical carcinoma was investigated. The presence of various HPV types was correlated with the histologic parameters of the carcinomas and with their immunoreactivity with antibodies to p53, Ki-67-Ag, and bcl-2.
Formalin fixed, paraffin embedded tissue specimens representing 159 cases of International Federation of Gynecology and Obstetrics Stage I and II were used. HPV DNA was detected by polymerase chain reaction (PCR) using a general primer set that targets the L1 region and synthesizes a product of only 65 base pairs. The HPV types were determined by direct sequencing and compared with known HPV types.
All 159 carcinomas were positive for HPV. HPV 16 (64.8%) was most frequently found, followed by HPV 18 (10.7%) and HPV 45 (8.2%). In 6 patients (3.8%), HPV types could not been further classified, and these cases were therefore categorized as HPV X. Although a trend was noted toward poorer prognosis for women with carcinomas harboring HPV types 16, 18, and 45 than for patients with carcinomas harboring HPV types 31, 33, 35, 52, 56, 58, and 68, the differences were not statistically significant. The prevalence of adenocarcinoma and adenosquamous carcinoma was higher among HPV 18 positive patients than among patients with the other known HPV types (P=0.0002).
The rate of HPV positivity in these 159 cervical carcinomas was 100%. These findings challenge the assumption that HPV negative cervical carcinomas exist. This high rate might be attributed to the use of a new broad-spectrum HPV PCR test. HPV typing in cervical carcinoma was not significantly related to clinical outcome. HPV 18 was significantly more frequently found in adenocarcinoma and adenosquamous carcinoma. The possibility of classifying HPV 45 as an oncogenic high risk type should be considered.
对159名俄罗斯宫颈癌女性患者人乳头瘤病毒（HPV）感染与肿瘤预后之间的相关性进行了研究。不同HPV类型的存在与癌组织学参数及其与p53、Ki-67抗原和bcl-2抗体的免疫反应性相关。
使用代表国际妇产科联盟（FIGO）I期和II期的159例病例的福尔马林固定、石蜡包埋组织标本。采用针对L1区域的通用引物组通过聚合酶链反应（PCR）检测HPV DNA，该引物组合成仅65个碱基对的产物。通过直接测序确定HPV类型，并与已知HPV类型进行比较。
所有159例癌组织HPV检测均为阳性。最常见的是HPV 16（64.8%），其次是HPV 18（10.7%）和HPV 45（8.2%）。6例患者（3.8%）的HPV类型无法进一步分类，因此这些病例归类为HPV X。尽管观察到携带HPV 16、18和45型癌的女性患者预后较携带HPV 31、33、35、52、56、58和68型癌的患者差，但差异无统计学意义。HPV 18阳性患者腺癌和腺鳞癌的患病率高于其他已知HPV类型的患者（P=0.0002）。
这159例宫颈癌的HPV阳性率为100%。这些发现对存在HPV阴性宫颈癌这一假设提出了挑战。这一高阳性率可能归因于使用了新的广谱HPV PCR检测方法。宫颈癌中的HPV分型与临床结局无显著相关性。HPV 18在腺癌和腺鳞癌中更常见。应考虑将HPV 45归类为致癌高危型的可能性。
