Papadopoulos N G, Sanderson G, Hunter J, Johnston S L
University Department of Medicine, University of Southampton, United Kingdom.
J Med Virol. 1999 May;58(1):100-4. doi: 10.1002/(sici)1096-9071(199905)58:1<100::aid-jmv16>3.0.co;2-d.
Rhinoviruses are epidemiologically connected to the majority of acute asthma exacerbations; however, their ability to infect and replicate in the lower airways is disputed. A frequent argument against this possibility involves the temperature preference for rhinovirus replication, generally accepted to be 33 degrees C, the temperature of the nasal passages. However, this argument is based on studies with a single rhinovirus serotype. In this study, differences in temperature preferences were evaluated between several serotypes and relative titers were determined than can be achieved at upper and lower airway temperatures. Rhinovirus serotypes 1b, 2, 7, 9, 14, 16, 41, and 70 were titrated in Ohio-HeLa cell cultures at either 33 degrees C or 37 degrees C. Possible selection by culture temperature was examined by continuous culture at 33 degrees C and 37 degrees C for 2-4 passages and subsequent titration at both temperatures. Finally, nasal aspirate samples derived from patients with wild-type rhinoviral common colds were cultured at 33 degrees C and 37 degrees C and RT-PCR was used to assess rhinovirus replication at each temperature. The majority of the serotypes and wild-type viruses replicated slightly better at 33 degrees C than at 37 degrees C. However, titers achieved after one or more replicative cycles at 37 degrees C were still high enough to initiate infection. Furthermore, in some instances equal or even better replication was observed at 37 degrees C. It is concluded that temperature preferences may vary between rhinoviruses and are not likely to be a prohibitive factor for infection of the lower airways.
鼻病毒在流行病学上与大多数急性哮喘加重发作相关；然而，它们在下呼吸道感染和复制的能力存在争议。反对这种可能性的一个常见论点涉及鼻病毒复制的温度偏好，一般认为是33摄氏度，即鼻腔通道的温度。然而，这个论点是基于对单一鼻病毒血清型的研究。在本研究中，评估了几种血清型之间的温度偏好差异，并确定了在上呼吸道和下呼吸道温度下可达到的相对滴度。鼻病毒血清型1b、2、7、9、14、16、41和70在俄亥俄-海拉细胞培养物中于33摄氏度或37摄氏度下进行滴定。通过在33摄氏度和37摄氏度下连续培养2 - 4代并随后在两个温度下进行滴定，研究了培养温度可能产生的选择作用。最后，将来自野生型鼻病毒普通感冒患者的鼻吸液样本在33摄氏度和37摄氏度下培养，并用逆转录-聚合酶链反应（RT-PCR）评估每个温度下的鼻病毒复制情况。大多数血清型和野生型病毒在33摄氏度下的复制略好于37摄氏度。然而，在37摄氏度下经过一个或多个复制周期后达到的滴度仍然高到足以引发感染。此外，在某些情况下，在37摄氏度下观察到了同等甚至更好的复制情况。结论是，鼻病毒之间的温度偏好可能有所不同，并且不太可能成为感染下呼吸道的阻碍因素。
