School of Medicine and Public Health, 600 Highland Avenue, University of Wisconsin, Madison, WI 53792, USA.
Institute for Molecular Virology, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA.
J Gen Virol. 2011 Nov;92(Pt 11):2549-2557. doi: 10.1099/vir.0.031302-0. Epub 2011 Jul 13.
HeLa cells are used to study the life cycles of many different viruses, including the human rhinoviruses (HRV) in the family Picornaviridae. Although the natural targets of HRV are human bronchial epithelial cells (hBE), it is generally more difficult to obtain and maintain the relevant primary cell cultures, relative to HeLa cells. Given that the HRV are now identified as a major cause of human asthma exacerbations, it becomes important to document how much of the virus biology learned from HeLa cells is common also to natural primary cells. When compared directly in matched infections using A01a virus, the kinetics of RNA replication, the synthesis and processing of viral proteins and the general subcellular localization of key non-structural proteins were resembled in hBE and HeLa cells. Viral-induced shutoff of host cell processes (e.g. nucleo-cytoplasmic trafficking) was also comparable.
HeLa 细胞被用于研究许多不同病毒的生命周期，包括小核糖核酸病毒科的人类鼻病毒（HRV）。尽管 HRV 的天然靶标是人支气管上皮细胞（hBE），但相对于 HeLa 细胞，获得和维持相关的原代细胞培养通常更为困难。鉴于 HRV 现已被确定为导致人类哮喘恶化的主要原因，记录从 HeLa 细胞中学到的病毒生物学有多少与天然原代细胞共同就变得很重要。当使用 A01a 病毒在匹配感染中直接进行比较时，RNA 复制、病毒蛋白的合成和加工以及关键非结构蛋白的一般亚细胞定位的动力学在 hBE 和 HeLa 细胞中相似。病毒诱导的宿主细胞过程（如核质运输）的关闭也是类似的。
