Leblanc N, Perrot-Rechenmann C, Barbier-Brygoo H
Perception and Auxin Transport, Institut des Sciences Végétales, CNRS, Gif sur Yvette, France.
FEBS Lett. 1999 Apr 16;449(1):57-60. doi: 10.1016/s0014-5793(99)00398-1.
Hyperpolarization of tobacco protoplasts is amongst the earliest auxin responses described. It has been proposed that the auxin-binding protein, ABP1, or a related protein could be involved in the first step of auxin perception at the plasma membrane. Using for the first time homologous conditions for interaction between the protein Nt-ERabp1 or a synthetic peptide corresponding to the C-terminus and tobacco protoplasts, we have demonstrated that both can induce the hyperpolarization response. The results show that Nt-ERabp1 or the C-terminal peptide alone activates the auxin pathway from the outer face of the plasma membrane.
烟草原生质体的超极化是最早被描述的生长素反应之一。有人提出，生长素结合蛋白ABP1或一种相关蛋白可能参与了生长素在质膜上感知的第一步。我们首次使用蛋白质Nt-ERabp1或对应于C末端的合成肽与烟草原生质体之间相互作用的同源条件，证明了两者都能诱导超极化反应。结果表明，单独的Nt-ERabp1或C末端肽可从质膜外表面激活生长素信号通路。
