Botanisches Institut und Botanischer Garten der Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
Plant Signal Behav. 2010 Jan;5(1):1-3. doi: 10.4161/psb.5.1.10306.
Auxin-binding protein 1 (ABP1) is an auxin receptor for responses not primarily regulated by gene regulation. One fast response is protoplast swelling. By using immunological ABP1 tools we showed that the highly conserved box a is not alone important for auxin binding. Box c is another part of the auxin binding domain.1 Here we present a novel method to analyze auxin-induced, ABP1-mediated effects at the plasma membrane on single cell level in vivo. The fluorescence of FM4-64 in the plasma membrane is reduced by auxin and this response is mediated by ABP1. This method indicates a functional role of ABP1 at the plasma membrane.
生长素结合蛋白 1(ABP1)是生长素受体,参与不主要受基因调控的反应。一个快速反应是原生质体膨胀。通过使用免疫 ABP1 工具,我们表明高度保守的框 a 对于生长素结合并不是唯一重要的。框 c 是生长素结合域的另一个部分。1 在这里,我们提出了一种新的方法来分析体内单细胞水平上生长素诱导的、由 ABP1 介导的质膜上的效应。质膜中的 FM4-64 荧光被生长素减少,这种反应是由 ABP1 介导的。该方法表明 ABP1 在质膜上具有功能作用。