Best T J, Mackinnon S E, Evans P J, Hunter D, Midha R
Institute of Medical Science, University of Toronto, Ontario, Canada.
J Reconstr Microsurg. 1999 Apr;15(3):183-90. doi: 10.1055/s-2007-1000090.
The revascularization of nerve grafts was investigated using histologic and morphometric techniques. Small-diameter nerve grafts (sciatic in the rat and sural in adult ewes) were studied, as was a large-diameter peroneal nerve graft in the ewe. Ninety-six hours after sciatic nerve engraftment, rats were injected with an intravascular fluorescent tracer. Evans blue albumin (EBA). Specimens were observed for the number of vessels perfused. Analysis showed no difference in vascular pattern between the grafted nerves and their control nerves, suggesting that spontaneous revascularization had occurred to establish a vascular tree essentially identical to the native nerve. Sural and peroneal nerve grafts were evaluated in adult ewes at 7 or 40 days post-nerve grafting. Similar to the rat sciatic nerve, the small-diameter sural nerve grafts were completely revascularized, with an equal number of perfused vessels at both time periods, with respect to control specimens. In contrast, the larger-caliber peroneal nerve grafts were not perfused at 7 days, and very poorly perfused at 40 days. This correlated with scant neural regeneration at 40 days. The finding suggests that small-diameter nerve grafts spontaneously revascularize, and revascularization using microvascular techniques is not necessary. In contrast, the larger-diameter nerve graft did not revascularize well. Such a large-diameter nerve graft would provide a suitable model to investigate the potential merits of a vascularized nerve graft.
采用组织学和形态计量学技术研究了神经移植物的血管再生情况。研究了小直径神经移植物（大鼠的坐骨神经和成年母羊的腓肠神经）以及母羊的大直径腓总神经移植物。坐骨神经植入96小时后，给大鼠注射血管内荧光示踪剂伊文思蓝白蛋白（EBA）。观察标本中灌注血管的数量。分析表明，移植神经与其对照神经的血管模式没有差异，这表明已发生自发血管再生，以建立与天然神经基本相同的血管树。在成年母羊神经移植后7天或40天对腓肠神经和腓总神经移植物进行评估。与大鼠坐骨神经相似，小直径的腓肠神经移植物完全实现了血管再生，在两个时间段内灌注血管的数量与对照标本相同。相比之下，较大口径的腓总神经移植物在7天时未灌注，在40天时灌注很差。这与40天时神经再生稀少相关。该发现表明，小直径神经移植物可自发实现血管再生，无需使用微血管技术进行血管再生。相比之下，较大直径的神经移植物血管再生不佳。这种大直径神经移植物将为研究血管化神经移植物的潜在优点提供一个合适的模型。
