Shi H Z, Li C Q, Qin S M, Xie Z F, Liu Y
Department of Internal Medicine, Guangxi Medical University, Nanning, Guangxi, 530021, People's Republic of China.
Clin Immunol. 1999 May;91(2):163-9. doi: 10.1006/clim.1999.4699.
The aim of the present study was to examine the effects of interleukin-5 (IL-5) inhalation on changes in the activity and number of circulating eosinophils, as well as concentrations of serum total IgE, in allergic asthmatics. A randomized double-blind, placebo-controlled study design was employed in which each subject acted as his or her own control. Eight nonsmoking patients with allergic asthma were administered recombinant human IL-5 by nebulization. Total white blood cell counts and differentials, as well as concentrations of ECP and total IgE in serum, were determined before and at 2, 24, and 48 h after inhalation. Our results demonstrated that eosinophil numbers increased from baseline (3.6 +/- 1.1 x 10(5)/ml) to 6.3 +/- 1.2 x 10(5)/ml (P < 0.01) at 24 h and to 5.7 +/- 0.9 x 10(5)/ml (P < 0.01) at 48 h after IL-5 inhalation in asthmatics. Accompanying this significantly increased blood eosinophilia were significantly elevated serum ECP levels. Compared with baseline (6.3 +/- 1.1 ng/ml), ECP levels increased with time following IL-5 inhalation, reaching 17.6 +/- 2.8 ng/ml (P < 0.01) at 24 h and remaining elevated at 48 h (18.1 +/- 2.9 ng/ml, P < 0.01). IL-5 inhalation had no significant effect on levels of serum total IgE, however. These findings provide direct evidence that nebulized IL-5 not only induces a significant blood eosinophilia but also results in the activation of circulating eosinophils. Our data further support the importance of IL-5 in the pathogenesis of bronchial asthma in humans.
本研究的目的是检测吸入白细胞介素-5（IL-5）对过敏性哮喘患者循环嗜酸性粒细胞活性和数量变化以及血清总IgE浓度的影响。采用随机双盲、安慰剂对照研究设计，每位受试者均作为自身对照。8名非吸烟过敏性哮喘患者通过雾化吸入重组人IL-5。在吸入前以及吸入后2、24和48小时测定外周血白细胞总数及分类，以及血清中嗜酸性粒细胞阳离子蛋白（ECP）和总IgE的浓度。我们的结果显示，哮喘患者吸入IL-5后，嗜酸性粒细胞数量在24小时时从基线水平（3.6±1.1×10⁵/ml）增加至6.3±1.2×10⁵/ml（P<0.01），48小时时增加至5.7±0.9×10⁵/ml（P<0.01）。伴随这种显著增加的血液嗜酸性粒细胞增多的是血清ECP水平显著升高。与基线水平（6.3±1.1 ng/ml）相比，吸入IL-5后ECP水平随时间升高，在24小时时达到17.6±2.8 ng/ml（P<0.01），并在48小时时保持升高（18.1±2.9 ng/ml，P<0.01）。然而，吸入IL-5对血清总IgE水平无显著影响。这些发现提供了直接证据，表明雾化吸入IL-5不仅可诱导显著的血液嗜酸性粒细胞增多，还可导致循环嗜酸性粒细胞活化。我们的数据进一步支持了IL-5在人类支气管哮喘发病机制中的重要性。
