Xu Feng, Zhang Hong-quan
Medical and Pharmacological, Institute of Yangzhou University, Yangzhou 225001, China.
Yao Xue Xue Bao. 2003 Sep;38(9):661-4.
To evaluate the effect of interleukin-1 receptor antagonist(IL-1ra) on apoptosis and associated mechanism of eosinophil in guinea pig with asthma.
A model of guinea pig with asthma was established. After inhalation of different concentrations of IL-1ra, asthma was induced in the guinea pig for 8 days, the concentration of eosinophil cationic protein (ECP) in serum and bronchoalveolar lavage fluid (BALF), IL-5 in serum, the eosinophil counts and apoptosis were assayed by radioimmunology, enzyme-linked immunosorbent assay(ELISA), fluoromicroscope and light microscope.
IL-1ra indirectly decreased the level of IL-5 in serum, improved the apoptosis of eosinophil(EOS) in lung, then decreased the level of ECP in serum and BALF.
Inhalation of nebulized IL-1ra showed protective effect against asthma through change of the activity and infiltration of EOS in lung.
评估白细胞介素-1受体拮抗剂（IL-1ra）对哮喘豚鼠嗜酸性粒细胞凋亡及相关机制的影响。
建立豚鼠哮喘模型。吸入不同浓度的IL-1ra后，诱发豚鼠哮喘8天，采用放射免疫法、酶联免疫吸附测定（ELISA）、荧光显微镜和光学显微镜检测血清及支气管肺泡灌洗液（BALF）中嗜酸性粒细胞阳离子蛋白（ECP）浓度、血清中IL-5、嗜酸性粒细胞计数及凋亡情况。
IL-1ra间接降低血清中IL-5水平，改善肺组织中嗜酸性粒细胞（EOS）凋亡，进而降低血清及BALF中ECP水平。
雾化吸入IL-1ra通过改变肺组织中EOS的活性和浸润情况对哮喘起到保护作用。
