Kozai K, Nakayama R, Tedjosasongko U, Kuwahara S, Suzuki J, Okada M, Nagasaka N
Department of Pediatric Dentistry, Hiroshima University School of Dentistry, Hiroshima, Japan.
Microbiol Immunol. 1999;43(2):99-106. doi: 10.1111/j.1348-0421.1999.tb02380.x.
The purpose of this study was to investigate the intrafamilial distribution of mutans streptococci in Japanese families using chromosomal DNA fingerprinting with three endonucleases; EcoRI, HindIII and HaeIII. The analysis of 1,908 isolates cultured from the dental plaque of 76 subjects from 20 families (20 married couples and 36 of their children) resulted in the identification of 144 genotypes containing 114 strains of Streptococcus mutans (serotype c, 66.7%; e, 12.5%) and 30 strains of S. sobrinus (d, 13.2%; g, 7.6%). A mean of 1.89 genotypes (from one to four) was harbored in individual subjects, and a mean of 4.10 genotypes from two to seven was harbored in individual families. Among the 70 genotypes found in the children, 36 (51.4%) were in agreement with their mothers and 22 (31.4%) were in agreement with their fathers. The other genotypes (18.6%) did not correspond with the parents. Homologous strains between parents were found in only two couples. This result showed that fathers or others as well as mothers can be sources of transmission. Further, the serotype d, e and g strains showed significantly higher probabilities of transmission than serotype c.
本研究的目的是使用三种核酸内切酶（EcoRI、HindIII和HaeIII）进行染色体DNA指纹分析，以调查日本家庭中变形链球菌的家庭内分布情况。对来自20个家庭（20对已婚夫妇及其36个孩子）的76名受试者牙菌斑中培养的1908株分离菌进行分析，结果鉴定出144种基因型，其中包括114株变形链球菌（血清型c，66.7%；e，12.5%）和30株远缘链球菌（血清型d，13.2%；g，7.6%）。个体受试者平均携带1.89种基因型（从1种到4种），个体家庭平均携带4.10种基因型（从2种到7种）。在儿童中发现的70种基因型中，36种（51.4%）与母亲一致，22种（31.4%）与父亲一致。其他基因型（18.6%）与父母不一致。仅在两对夫妇中发现了父母之间的同源菌株。这一结果表明，父亲或其他人和母亲一样可能是传播源。此外，血清型d、e和g菌株的传播概率明显高于血清型c。
