分离淀粉颗粒中的颗粒结合型淀粉合酶I持续延长麦芽寡糖。
Granule-bound starch synthase I in isolated starch granules elongates malto-oligosaccharides processively.
作者信息
Denyer K, Waite D, Motawia S, Møller B L, Smith A M
机构信息
John Innes Centre, Norwich Research Park, Norwich, Norfolk NR4 7UH, UK.
出版信息
Biochem J. 1999 May 15;340 ( Pt 1)(Pt 1):183-91.
Abstract
Isoforms of starch synthase belonging to the granule-bound starch synthase I (GBSSI) class synthesize the amylose component of starch in plants. Other granule-bound isoforms of starch synthase, such as starch synthase II (SSII), are unable to synthesize amylose. The kinetic properties of GBSSI and SSII that are responsible for these functional differences have been investigated using starch granules from embryos of wild-type peas and rug5 and lam mutant peas, which contain, respectively, both GBSSI and SSII, GBSSI but not SSII and SSII but not GBSSI. We show that GBSSI in isolated granules elongates malto-oligosaccharides processively, adding more than one glucose molecule for each enzyme-glucan encounter. Granule-bound SSII can elongate malto-oligosaccharides, but has a lower affinity for these than GBSSI and does not elongate processively. As a result of these properties GBSSI synthesizes longer malto-oligosaccharides than SSII. The significance of these results with respect to the roles of GBSSI and SSII in vivo is discussed.
摘要
属于颗粒结合型淀粉合酶I（GBSSI）类别的淀粉合酶同工型在植物中合成淀粉的直链淀粉成分。其他颗粒结合型淀粉合酶同工型，如淀粉合酶II（SSII），则无法合成直链淀粉。利用来自野生型豌豆以及rug5和lam突变体豌豆胚的淀粉颗粒，分别对导致这些功能差异的GBSSI和SSII的动力学特性进行了研究，这些豌豆胚分别含有GBSSI和SSII、仅含有GBSSI而不含SSII以及仅含有SSII而不含GBSSI。我们发现，分离颗粒中的GBSSI持续延长麦芽寡糖，每次酶与葡聚糖相遇时添加不止一个葡萄糖分子。颗粒结合型SSII能够延长麦芽寡糖，但对其亲和力低于GBSSI，且不能持续延长。由于这些特性，GBSSI合成的麦芽寡糖比SSII更长。文中讨论了这些结果对于GBSSI和SSII在体内作用的意义。
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