马铃薯块茎可溶性部分中主要淀粉合酶的鉴定。
Identification of the major starch synthase in the soluble fraction of potato tubers.
作者信息
Marshall J, Sidebottom C, Debet M, Martin C, Smith A M, Edwards A
机构信息
John Innes Centre, Norwich, United Kingdom.
出版信息
Plant Cell. 1996 Jul;8(7):1121-35. doi: 10.1105/tpc.8.7.1121.
Abstract
The major isoform of starch synthase from the soluble fraction of developing potato tubers has been purified and used to prepare an antibody and isolate a cDNA. The protein is 140 kD, and it is distinctly different in predicted primary amino acid sequence from other isoforms of the enzyme thus far described. Immunoinhibition and immunoblotting experiments and analysis of tubers in which activity of the isoform was reduced through expression of antisense mRNA revealed that the isoform accounts for approximately 80% of the activity in the soluble fraction of the tuber and that it is also bound to starch granules. Severe reductions in activity had no discernible effect on starch content or amylose-to-amylopectin ratio of starch in tubers. However, they caused a profound change in the morphology of starch granules, indicative of important underlying changes in the structure of starch polymers within the granule.
摘要
已从发育中的马铃薯块茎可溶性部分纯化出淀粉合酶的主要同工型，并用于制备抗体和分离cDNA。该蛋白质为140 kD，其预测的一级氨基酸序列与迄今为止描述的该酶的其他同工型明显不同。免疫抑制和免疫印迹实验以及对通过反义mRNA表达降低同工型活性的块茎的分析表明，该同工型约占块茎可溶性部分活性的80%，并且它也与淀粉颗粒结合。活性的严重降低对块茎中淀粉含量或淀粉的直链淀粉与支链淀粉比例没有明显影响。然而，它们导致淀粉颗粒形态发生深刻变化，这表明颗粒内淀粉聚合物结构发生了重要的潜在变化。
相似文献
1
Identification of the major starch synthase in the soluble fraction of potato tubers.马铃薯块茎可溶性部分中主要淀粉合酶的鉴定。
Plant Cell. 1996 Jul;8(7):1121-35. doi: 10.1105/tpc.8.7.1121.
2
Biochemical and molecular characterization of a novel starch synthase from potato tubers.马铃薯块茎中一种新型淀粉合酶的生化与分子特性
Plant J. 1995 Aug;8(2):283-94. doi: 10.1046/j.1365-313x.1995.08020283.x.
3
Role of granule-bound starch synthase in determination of amylopectin structure and starch granule morphology in potato.颗粒结合型淀粉合酶在马铃薯支链淀粉结构和淀粉颗粒形态决定中的作用
J Biol Chem. 2002 Mar 29;277(13):10834-41. doi: 10.1074/jbc.M111579200. Epub 2002 Jan 18.
4
Cloning and functional analysis of a cDNA encoding a starch synthase from potato (Solanum tuberosum L.) that is predominantly expressed in leaf tissue.一个主要在叶片组织中表达的马铃薯（Solanum tuberosum L.）淀粉合酶编码cDNA的克隆与功能分析
Planta. 1999 Jun;208(4):503-11. doi: 10.1007/s004250050587.
5
Isolation and characterization of a cDNA encoding granule-bound starch synthase in cassava (Manihot esculenta Crantz) and its antisense expression in potato.木薯（Manihot esculenta Crantz）中编码颗粒结合型淀粉合成酶的cDNA的分离、鉴定及其在马铃薯中的反义表达
Plant Mol Biol. 1993 Dec;23(5):947-62. doi: 10.1007/BF00021811.
6
Specificity of starch synthase isoforms from potato.马铃薯淀粉合酶同工型的特异性
Eur J Biochem. 1999 Dec;266(3):724-36. doi: 10.1046/j.1432-1327.1999.00861.x.
7
Cloning and functional analysis of a cDNA encoding a novel 139 kDa starch synthase from potato (Solanum tuberosum L.).来自马铃薯（Solanum tuberosum L.）的一种编码新型139 kDa淀粉合酶的cDNA的克隆与功能分析。
Plant J. 1996 Dec;10(6):981-91. doi: 10.1046/j.1365-313x.1996.10060981.x.
8
Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs.通过反义构建体抑制马铃薯中颗粒结合淀粉合成酶基因的表达。
Mol Gen Genet. 1991 Feb;225(2):289-96. doi: 10.1007/BF00269861.
9
Field evaluation of transgenic potato plants expressing an antisense granule-bound starch synthase gene: increase of the antisense effect during tuber growth.表达反义颗粒结合淀粉合成酶基因的转基因马铃薯植株的田间评价：块茎生长期间反义效应的增强
Plant Mol Biol. 1994 Dec;26(6):1759-73. doi: 10.1007/BF00019490.
10
A second L-type isozyme of potato glucan phosphorylase: cloning, antisense inhibition and expression analysis.马铃薯葡聚糖磷酸化酶的第二种L型同工酶：克隆、反义抑制及表达分析。
Plant Mol Biol. 1995 Feb;27(3):567-76. doi: 10.1007/BF00019322.
引用本文的文献
1
Exploring regulatory networks in plants: transcription factors of starch metabolism.探索植物中的调控网络：淀粉代谢的转录因子
PeerJ. 2019 Jul 9;7:e6841. doi: 10.7717/peerj.6841. eCollection 2019.
2
Potato starch synthases: Functions and relationships.马铃薯淀粉合酶：功能与关系
Biochem Biophys Rep. 2017 Feb 20;10:7-16. doi: 10.1016/j.bbrep.2017.02.004. eCollection 2017 Jul.
3
Genome-wide analysis of starch metabolism genes in potato (Solanum tuberosum L.).马铃薯（Solanum tuberosum L.）淀粉代谢基因的全基因组分析。
BMC Genomics. 2017 Jan 5;18(1):37. doi: 10.1186/s12864-016-3381-z.
4
Formation of starch in plant cells.植物细胞中淀粉的形成。
Cell Mol Life Sci. 2016 Jul;73(14):2781-807. doi: 10.1007/s00018-016-2250-x. Epub 2016 May 11.
5
Genetic Evidence That Chain Length and Branch Point Distributions Are Linked Determinants of Starch Granule Formation in Arabidopsis.链长和分支点分布是拟南芥淀粉颗粒形成的相关决定因素的遗传学证据。
Plant Physiol. 2014 Aug;165(4):1457-1474. doi: 10.1104/pp.114.241455. Epub 2014 Jun 25.
6
Transcriptome assembly and analysis of Tibetan Hulless Barley (Hordeum vulgare L. var. nudum) developing grains, with emphasis on quality properties.青稞（裸大麦）发育籽粒的转录组组装与分析，重点关注品质特性。
PLoS One. 2014 May 28;9(5):e98144. doi: 10.1371/journal.pone.0098144. eCollection 2014.
7
Genetic controls on starch amylose content in wheat and rice grains.小麦和水稻籽粒中淀粉直链淀粉含量的遗传控制
J Genet. 2014 Apr;93(1):279-92. doi: 10.1007/s12041-014-0325-8.
8
Starch biosynthesis in rice endosperm requires the presence of either starch synthase I or IIIa.水稻胚乳中的淀粉合成需要淀粉合酶 I 或 IIIa 的存在。
J Exp Bot. 2011 Oct;62(14):4819-31. doi: 10.1093/jxb/err125. Epub 2011 Jul 5.
9
Starch biosynthetic enzymes from developing maize endosperm associate in multisubunit complexes.来自发育中的玉米胚乳的淀粉生物合成酶以多亚基复合物的形式结合。
Plant Physiol. 2008 Apr;146(4):1892-908. doi: 10.1104/pp.108.116285. Epub 2008 Feb 15.
10
Characterization of SSIIIa-deficient mutants of rice: the function of SSIIIa and pleiotropic effects by SSIIIa deficiency in the rice endosperm.水稻SSIIIa缺陷型突变体的表征：SSIIIa在水稻胚乳中的功能及SSIIIa缺陷的多效性影响
Plant Physiol. 2007 Aug;144(4):2009-23. doi: 10.1104/pp.107.102533. Epub 2007 Jun 22.
本文引用的文献
1
Identification of granule-bound starch synthase in potato tubers.马铃薯块茎中颗粒结合型淀粉合酶的鉴定
Plant Physiol. 1986 Oct;82(2):411-6. doi: 10.1104/pp.82.2.411.
2
Modifying starch biosynthesis with transgenes in potatoes.利用转基因技术改良马铃薯淀粉生物合成过程。
Plant Physiol. 1992 Nov;100(3):1083-6. doi: 10.1104/pp.100.3.1083.
3
Formation and Deposition of Amylose in the Potato Tuber Starch Granule Are Affected by the Reduction of Granule-Bound Starch Synthase Gene Expression.马铃薯块茎淀粉粒中直链淀粉的形成与沉积受颗粒结合型淀粉合酶基因表达降低的影响。
Plant Cell. 1994 Jan;6(1):43-52. doi: 10.1105/tpc.6.1.43.
4
What Controls the Amount and Structure of Starch in Storage Organs?是什么控制着贮藏器官中淀粉的数量和结构？
Plant Physiol. 1995 Mar;107(3):673-677. doi: 10.1104/pp.107.3.673.
5
Isolation and characterization of a cDNA encoding granule-bound starch synthase in cassava (Manihot esculenta Crantz) and its antisense expression in potato.木薯（Manihot esculenta Crantz）中编码颗粒结合型淀粉合成酶的cDNA的分离、鉴定及其在马铃薯中的反义表达
Plant Mol Biol. 1993 Dec;23(5):947-62. doi: 10.1007/BF00021811.
6
Soluble isoforms of starch synthase and starch-branching enzyme also occur within starch granules in developing pea embryos.淀粉合酶和淀粉分支酶的可溶性同工型也存在于发育中的豌豆胚胎的淀粉粒中。
Plant J. 1993 Jul;4(1):191-8. doi: 10.1046/j.1365-313x.1993.04010191.x.
7
Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation.枯草芽孢杆菌中的糖原：参与糖原生物合成与降解的酶编码操纵子的分子特征
Mol Microbiol. 1994 Jan;11(1):203-18. doi: 10.1111/j.1365-2958.1994.tb00301.x.
8
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
9
Binary Agrobacterium vectors for plant transformation.用于植物转化的二元农杆菌载体。
Nucleic Acids Res. 1984 Nov 26;12(22):8711-21. doi: 10.1093/nar/12.22.8711.
10
Studies on the biosynthesis of starch. I. Isolation and properties of the soluble adenosine diphosphate glucose: starch glucosyltransferase of Solanum tuberosum.淀粉生物合成的研究。I. 马铃薯可溶性二磷酸腺苷葡萄糖：淀粉葡糖基转移酶的分离与性质
Arch Biochem Biophys. 1966 Sep 26;116(1):9-18. doi: 10.1016/0003-9861(66)90005-1.
Zotero 插件