从刀豆中提取的α-甘露糖苷酶反向作用产生的二甘露糖基和三甘露糖基环麦芽七糖（β-环糊精）的分离与表征

Isolation and characterization of di- and tri-mannosyl-cyclomaltoheptaoses (beta-cyclodextrins) produced by reverse action of alpha-mannosidase from jack bean.

作者信息

Koizumi K, Tanimoto T, Okada Y, Takeyama S, Hamayasu K, Hashimoto H, Kitahata S

机构信息

School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.

出版信息

Carbohydr Res. 1998 Dec 21;314(1-2):115-25. doi: 10.1016/s0008-6215(98)00267-5.

DOI:10.1016/s0008-6215(98)00267-5

PMID:10230040

Abstract

Di- and tri-mannosyl-cyclomaltoheptaoses (beta-cyclodextrins, beta CDs), which were synthesized together with monomannosyl-beta CD in a large-scale production by reverse action of alpha-mannosidase from jack bean, were isolated and purified by HPLC. The structures of seven isomers of di-mannosyl-beta CD and six isomers of tri-mannosyl-beta CD were elucidated by FABMS and NMR spectroscopy, and by enzymatic methods.

摘要

通过刀豆α-甘露糖苷酶的逆反应大规模生产得到的二甘露糖基和三甘露糖基环麦芽七糖（β-环糊精，β-CD），与单甘露糖基-β-CD一起合成，通过高效液相色谱法进行分离和纯化。通过快原子轰击质谱（FABMS）、核磁共振光谱法以及酶法阐明了二甘露糖基-β-CD的七种异构体和三甘露糖基-β-CD的六种异构体的结构。