来自刀豆和杏仁的α-甘露糖苷酶的受体特异性以及支链环糊精的转甘露糖基作用。

Acceptor specificities of alpha-mannosidases from jack bean and almond, and transmannosylation of branched cyclodextrins.

作者信息

Hara K, Fujita K, Nakano H, Kuwahara N, Tanimoto T, Hashimoto H, Koizumi K, Kitahata S

机构信息

Osaka Municipal Technical Research Institute, Japan.

出版信息

Biosci Biotechnol Biochem. 1994 Jan;58(1):60-3. doi: 10.1271/bbb.58.60.

DOI:10.1271/bbb.58.60

PMID:7764520

Abstract

Jack bean alpha-mannosidase had a wide acceptor specificity and could transfer mannosyl residues to various acceptors such as D-fructose, L-arabinose, maltose, lactose, and sucrose. The structures of the transferred products of branched cyclodextrins (CDs) (glucosyl-beta CD, maltosyl-alpha CD, and maltosyl-beta CD) were found to be alpha-D-mannosyl-(1-->6)-alpha-D-glucosyl-(1-->6)-beta CD, alpha-D-mannosyl- (1-->6)-alpha-D-glucosyl-(1-->4)-alpha-D-glucosyl-(1-->6)-alpha CD and alpha-D-mannosyl-(1-->6)-alpha-D-glucosyl-(1-->4)-alpha-D-glucosyl-(1--> 6)- beta CD, respectively. Almond alpha-mannosidase also produced the same transmannosylated products of branched CDs.

摘要

刀豆α-甘露糖苷酶具有广泛的受体特异性，能够将甘露糖基残基转移至多种受体，如D-果糖、L-阿拉伯糖、麦芽糖、乳糖和蔗糖。研究发现，支链环糊精（CDs）（葡萄糖基-β-CD、麦芽基-α-CD和麦芽基-β-CD）的转糖基化产物结构分别为α-D-甘露糖基-（1→6）-α-D-葡萄糖基-（1→6）-β-CD、α-D-甘露糖基-（1→6）-α-D-葡萄糖基-（1→4）-α-D-葡萄糖基-（1→6）-α-CD和α-D-甘露糖基-（1→6）-α-D-葡萄糖基-（1→4）-α-D-葡萄糖基-（1→6）-β-CD。杏仁α-甘露糖苷酶也能产生相同的支链环糊精转甘露糖基化产物。