Gallimore M J, Jones D W, Winter M
Haemophilia Centre, Kent and Canterbury Hospitals NHS Trust, United Kingdom.
Thromb Haemost. 1998 Jan;79(1):87-90.
Factor XII (FXII) levels were determined in plasma samples from 29 normal donors, 10 patients with inherited FXII deficiency (all lupus anticoagulant [LA] negative) and 67 LA positive patients, using clotting (FXIIct), chromogenic substrate (FXIIcs) and immunochemical (FXIIag) assays. Excellent correlations were obtained in the three FXII assays with the LA negative samples and between the FXIIcs and FXIIag assays in the LA positive samples. Correlations between both the FXIIcs and FXIIag with FXIIct in the LA positive patients were poor. Of 67 LA positive samples studied, 25 (37.3%) showed lower values in the FXIIct assay; 13 (19.4%) of these patients were pseudo FXII deficient with values of FXII below the lower limit of normal. These results indicate that a diagnosis of FXII deficiency can be made inappropriately in the presence of phospholipid antibodies and that such a diagnosis should not be made by FXIIct assay alone.
采用凝血法（FXIIct）、发色底物法（FXIIcs）和免疫化学法（FXIIag）检测了29名正常献血者、10名遗传性FXII缺乏症患者（均为狼疮抗凝物[LA]阴性）和67名LA阳性患者血浆样本中的FXII水平。在LA阴性样本的三种FXII检测方法之间以及LA阳性样本的FXIIcs和FXIIag检测方法之间获得了良好的相关性。LA阳性患者中，FXIIcs和FXIIag与FXIIct之间的相关性较差。在研究的67份LA阳性样本中，25份（37.3%）在FXIIct检测中显示较低值；其中13名（19.4%）患者为假性FXII缺乏，FXII值低于正常下限。这些结果表明，在存在磷脂抗体的情况下，可能会不恰当地诊断为FXII缺乏，且不应仅通过FXIIct检测进行此类诊断。
