Zhang P F, Chen X, Fu D W, Margolick J B, Quinnan G V
Department of Preventive Medicine and Biometrics, Division of Tropical Public Health, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
J Virol. 1999 Jun;73(6):5225-30. doi: 10.1128/JVI.73.6.5225-5230.1999.
To test the hypothesis that changing neutralizing antibody responses against human immunodeficiency virus type 1 (HIV-1) during chronic infection were a response to emergence of neutralization escape mutants, we cloned expressed and characterized envelope clones from patients in the Multicenter AIDS Cohort Study (MACS). Pseudotyped HIV-1 envelope clones obtained from differing time points were assessed for sensitivity to neutralization by using sera from different times from the same and different patients. Clones from early and late time points during chronic infection had similar neutralization sensitivity, and neutralizing antibody responses cross-reacted with early, late, and heterologous envelopes. The potential for broadly effective HIV-1 immunization is supported.
为了验证慢性感染期间针对1型人类免疫缺陷病毒（HIV-1）的中和抗体反应变化是对中和逃逸突变体出现的反应这一假设，我们在多中心艾滋病队列研究（MACS）中克隆、表达并鉴定了来自患者的包膜克隆。使用来自同一患者和不同患者不同时间的血清，评估从不同时间点获得的假型HIV-1包膜克隆的中和敏感性。慢性感染早期和晚期时间点的克隆具有相似的中和敏感性，并且中和抗体反应与早期、晚期和异源包膜发生交叉反应。这支持了广泛有效的HIV-1免疫的可能性。
