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1H NMR study of the conversion of 13(S)-hydroperoxy linoleic acid by soya bean lipoxygenase I.

作者信息

Egmond M R, Williams R J

出版信息

Biochim Biophys Acta. 1978 Nov 22;531(2):141-8. doi: 10.1016/0005-2760(78)90137-6.

DOI:10.1016/0005-2760(78)90137-6
PMID:102362
Abstract

The conversion of 13(S)-hydroperoxy linoleic acid by lipoxygenase I at 298 K was monitored by 1H NMR and ultraviolet absorption spectroscopy. The rate constant for the conversion of the hydroperoxide, k = 45.8 +/- 7.5 M-1 . s-1, depends on the concentrations of both enzyme and hydroperoxide. This constant is not affected by O2, nor by solvent isotope effects.

摘要

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1H NMR study of the conversion of 13(S)-hydroperoxy linoleic acid by soya bean lipoxygenase I.
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