Kompiang I P, Gibson W R
Horm Metab Res. 1976 Sep;8(5):340-5. doi: 10.1055/s-0028-1093629.
Hypophysectomy increases hepatic lipogenesis in cockerels. In an attempt to find the possible hormonal mechanism for this we have examined the effects of hypophysectomy, insulin and pituitary homogenates on hepatic lipogenesis. Insulin (5 U/kg) injected intravenously, simultaneously with glucose-14C tracer, increased the amount of 14C found in liver lipids at 30 min after the injection. Similarly, insulin injected 5 min before killing, increased the incorporation of glucose-14C and acetate-14C by liver slices during a 30 min incubation. Hypophysectomy increased lipogenesis within 24 hours. The effect of insulin was most pronounced in hypophysectomized cockerels. The activity of the lipogenic enzyme, acetyl CoA carboxylase was similarly affected. A homogenate of chicken pituitaries added to the medium reduced lipid synthesis from glucose-14C by liver slices. This effect was larger in liver slices in which lipogenesis had been stimulated by insulin. The increased rate of hepatic lipgenesis in hypophysectomized cockerels may be caused partly by increased hepatic sensitivity to the lipogenic action of insulin or by the removal of a direct inhibition by pituitary hormones.
垂体切除可增加小公鸡的肝脏脂肪生成。为了探寻其可能的激素机制，我们研究了垂体切除、胰岛素和垂体匀浆对肝脏脂肪生成的影响。静脉注射胰岛素（5 U/kg）并同时注入葡萄糖 - 14C示踪剂，注射后30分钟时肝脏脂质中14C的含量增加。同样，在处死前5分钟注射胰岛素，可增加肝切片在30分钟孵育期间葡萄糖 - 14C和乙酸盐 - 14C的掺入量。垂体切除在24小时内增加了脂肪生成。胰岛素的作用在垂体切除的小公鸡中最为明显。脂肪生成酶乙酰辅酶A羧化酶的活性也受到类似影响。向培养基中添加鸡垂体匀浆可减少肝切片由葡萄糖 - 14C合成脂质。这种作用在胰岛素刺激脂肪生成的肝切片中更大。垂体切除的小公鸡肝脏脂肪生成速率增加，可能部分是由于肝脏对胰岛素脂肪生成作用的敏感性增加，或由于垂体激素直接抑制作用的消除。
