植物细胞悬浮培养物中烟酸的代谢：II；烟酸N-α-阿拉伯糖苷的分离、表征及酶学（作者译）

[Metabolism of nicotinic acid in plant cell suspension cultures: II; Isolation, characterization and enzymology of nicotinic acid N-alpha-arabinoside (author's transl)].

作者信息

Leienbach K W, Barz W

出版信息

Hoppe Seylers Z Physiol Chem. 1976 Aug;357(8):1069-80.

Abstract

A very hydrophilic compound was isolated from parsley cell suspension cultures in high yield after application of nicotinic acid. Using chemical, chromatographic and spectroscopic procedures the structure of this new plant constituent has been elucidated as nicotinic acid N-alpha-L-arabinopyranoside. This structure has been proved by chemical synthesis. An arabinosyltransferase was isolated from parsley cell suspension cultures and purified about 19-fold. The enzyme converted nicotinic acid N-alpha-arabinoside with UDP to nicotinic acid and UDP-arabinose. pH-Optimum (pH 7.0-8.0), Km value for nicotinic acid N-alpha-L-arabinoside (2.2 X 10(-4) mol/l) and mol. wt. (app. 70 000) of the transferase were measured. Function and biosynthesis of the arabinoside in cell cultures are discussed.

摘要

在施加烟酸后，从欧芹细胞悬浮培养物中高产率地分离出一种亲水性很强的化合物。通过化学、色谱和光谱方法，已阐明这种新植物成分的结构为烟酸N-α-L-阿拉伯吡喃糖苷。该结构已通过化学合成得到证实。从欧芹细胞悬浮培养物中分离出一种阿拉伯糖基转移酶，并将其纯化了约19倍。该酶将烟酸N-α-阿拉伯糖苷与UDP转化为烟酸和UDP-阿拉伯糖。测定了转移酶的最适pH（pH 7.0 - 8.0）、烟酸N-α-L-阿拉伯糖苷的Km值（2.2×10⁻⁴ mol/l）和分子量（约70000）。讨论了细胞培养物中阿拉伯糖苷的功能和生物合成。