Belova V S, Gvozdev R I, Malashenko I U, Sadkov A P, Iurchenko V V
Biokhimiia. 1976 Oct;41(10):1903-4.
The isotopic effect during oxidation of methane and deuteromethane by a suspension of Methylomonas rubrum cells, for which methane is the only source of carbon, was observed. The rate of CH4 oxidation is 12.5 times higher than that of CH4 oxidation. It is demonstrated that CD4 is a competitive inhibitor of CH4 oxidation. The results obtained suggest that the disruption of the C-H bond is the limiting step of enzymatic oxidation of methane.
观察了以甲烷作为唯一碳源的红甲基单胞菌细胞悬浮液对甲烷和氘代甲烷氧化过程中的同位素效应。CH₄的氧化速率比CD₄的氧化速率高12.5倍。结果表明，CD₄是CH₄氧化的竞争性抑制剂。所得结果表明，C-H键的断裂是甲烷酶促氧化的限速步骤。
