将商用放射免疫分析试剂盒的检测结果与血清和全血中叶酸的微生物学检测结果进行比较。

Results with commercial radioassay kits compared with microbiological assay of folate in serum and whole-blood.

作者信息

McGown E L, Lewis C M, Dong M H, Sauberlich H E

出版信息

Clin Chem. 1978 Dec;24(12):2186-91.

Abstract

We compared results with three commercial folate radioassay kits [Bio-Rad, New England Nuclear (NEN), and RIA Products] with those by microbiological assay for more than 200 samples of human serum and whole blood. All but one kit (NEN) compared favorably with the microbiological assay for serum samples, although there were notable diagnostic discrepancies. Two kits (NEN and Bio-Rad) were tested on whole-blood samples; both yielded values significantly higher than those by microbiological assay. The frequency distributions of erythrocyte folate data differed strikingly between the two kits; the NEN method yielded a much narrower range of normal values than did either the Bio-Rad or the microbiological assay. Radioassay kits appear to be suitable diagnostic agents for serum folate, if the behavior of a particular kit is investigated thoroughly before its routine use. However, the diagnostic value of radioassays of erythrocyte folate needs to be validated.

摘要

我们将三种商用叶酸放射免疫分析试剂盒[伯乐公司（Bio-Rad）、新英格兰核公司（New England Nuclear，NEN）和放射免疫分析产品公司（RIA Products）]的检测结果与微生物学检测法对200多个血清和全血样本的检测结果进行了比较。除了一种试剂盒（NEN）外，其他所有试剂盒对血清样本的检测结果与微生物学检测法相比都不错，不过存在明显的诊断差异。两种试剂盒（NEN和Bio-Rad）用于全血样本检测；二者得出的值均显著高于微生物学检测法所得的值。两种试剂盒的红细胞叶酸数据频率分布差异显著；NEN法得出的正常值范围比Bio-Rad法或微生物学检测法的范围窄得多。如果在常规使用前对特定试剂盒的性能进行全面研究，放射免疫分析试剂盒似乎是血清叶酸合适的诊断试剂。然而，红细胞叶酸放射免疫分析的诊断价值需要得到验证。