Brown R D, Robin H, Kronenberg H
Pathology. 1982 Oct;14(4):449-53. doi: 10.3109/00313028209092126.
A simple and reliable folate radioassay technique based on the principles of classic folate radioassays but incorporating several of the recently introduced technical innovations is presented as an alternative to the microbiological assays and commercial kits. Serum and red cell folate values from this method were compared with results from two commercial kits and a Lactobacillus casei (L. casei) assay. There was a good correlation with the L. casei assay for both serum and red cell folate (r = 0.95). The method uses N-methyltetrahydrofolate standards diluted in folate free serum, beta-lactoglobulin as a binder and an alkaline denaturation ('no-boil') of endogenous folate binding proteins. Low hematocrit lysates were best performed at a 1 in 10 rather than 1 in 20 dilution. A comparison of the boil and 'no-boil' kits from Diagnostic Products Corporation revealed that the 'no-boil' kit produced results closer to those obtained from the L. casei assay than the boil kit.
本文介绍了一种简单可靠的叶酸放射分析技术，该技术基于经典叶酸放射分析原理，但融入了一些最近引入的技术创新，可作为微生物分析和商业试剂盒的替代方法。将该方法测得的血清和红细胞叶酸值与两种商业试剂盒以及干酪乳杆菌（L. casei）分析结果进行了比较。血清和红细胞叶酸与干酪乳杆菌分析均具有良好的相关性（r = 0.95）。该方法使用在无叶酸血清中稀释的N-甲基四氢叶酸标准品、β-乳球蛋白作为结合剂，并对内源性叶酸结合蛋白进行碱性变性（“不煮沸”）处理。低血细胞比容裂解物在1:10稀释而非1:20稀释时效果最佳。对诊断产品公司的煮沸试剂盒和“不煮沸”试剂盒进行比较发现，“不煮沸”试剂盒产生的结果比煮沸试剂盒更接近干酪乳杆菌分析获得的结果。
