[Enzymatic determination of total serum cholesterol by 4-aminophenazone-phenol: manual and automatic method (author's transl)].

A direct method is proposed for total serum cholesterol determination by two specific enzymes and a coupled colorimetric reaction according to Trinder. The red colored compound is antipyridilquinonimine. The method is simple (serum plus reagent), rapid (30 min of incubation) and relatively inexpensive. It is perfectly correlated with Röschlau's method and easy to apply to discrete automatic methods.