Savoldi R, Prandini B D, Donisi C
Quad Sclavo Diagn. 1976 Jun;12(2):238-47.
A direct method is proposed for total serum cholesterol determination by two specific enzymes and a coupled colorimetric reaction according to Trinder. The red colored compound is antipyridilquinonimine. The method is simple (serum plus reagent), rapid (30 min of incubation) and relatively inexpensive. It is perfectly correlated with Röschlau's method and easy to apply to discrete automatic methods.
本文提出了一种直接法，通过两种特定酶和基于Trinder反应的偶联比色反应来测定血清总胆固醇。生成的红色化合物是安替比林醌亚胺。该方法简单（血清加试剂）、快速（孵育30分钟）且成本相对较低。它与罗施劳法具有良好的相关性，并且易于应用于离散自动分析方法。
