Stähler F, Munz E, Kattermann R
Dtsch Med Wochenschr. 1975 Apr 18;100(16):876-80, 885-7. doi: 10.1055/s-0028-1106309.
Cholesterol can be specifically measured without difficulty and without complex reagents by means of a newly developed enzymatic colour test. Intensive technical evaluation confirmed its accuracy. Manual use gave a day-to-day coefficient of variation of 2-3 per cent; the sensitivity at a cholesterol concentration of 200 mg/dl was E equals 0.153 (gamma equals 405 nm), with a linearity up to 1000 mg/dl. Recovery of added pure cholesterol solution was 100 plus or minus 2 percent. A quantitative study of 53 representative drugs, anti-coagulants and metabolites was performed both in test tube and on patients. Accuracy of the result was unaffected by any of the substances (alpha equals 0.05). Comparison with the multi-step extraction method used at present as a reference (Abell-Kendall) gave a regression equation of y equals 0.99 times plus 1.1 (x axis: extraction method; y axis: enzymatic colour test). The direct chemical method after Liebermann and Burchard gave, in part, markedly differing results because of considerable systematic and accidental errors.
通过一种新开发的酶促比色法可以轻松且无需使用复杂试剂地特异性测定胆固醇。深入的技术评估证实了其准确性。手动操作时，每日变异系数为2% - 3%；在胆固醇浓度为200mg/dl时，灵敏度为E = 0.153（γ = 405nm），线性范围高达1000mg/dl。添加的纯胆固醇溶液回收率为100±2%。对53种代表性药物、抗凝剂和代谢物进行了试管内和患者身上的定量研究。结果的准确性不受任何一种物质的影响（α = 0.05）。与目前用作参考的多步萃取法（阿贝尔 - 肯德尔法）比较，得到回归方程y = 0.99x + 1.1（x轴：萃取法；y轴：酶促比色法）。利伯曼和伯查德之后的直接化学法部分结果因存在相当大的系统误差和偶然误差而明显不同。
