Malaspina J P, Fourche J, Jensen H, Neuzil E
Ann Biol Clin (Paris). 1980;38(4):207-13.
The authors describe a new method of cholesterol determination, using the intense blue color given by interaction with 2,3-naphthalenedicarboxaldehyde in a sulphoacetic medium. The degree of precision of the method, as well as its reproducibility are discussed; a good correlation of the new method with two classical techniques, the colorimetric method of Liebermann and the enzymatic method, is observed. The high sensitivity and the specificity of the colour reaction allow the choice of the method for a direct determination of serum cholesterol using a 10 microliter sample.
作者描述了一种新的胆固醇测定方法，该方法利用在磺基乙酸介质中与2,3-萘二甲醛相互作用产生的强烈蓝色。讨论了该方法的精密度及其重现性；观察到该新方法与两种经典技术（利伯曼比色法和酶法）具有良好的相关性。该显色反应的高灵敏度和特异性使得可以选择该方法使用10微升样品直接测定血清胆固醇。
