Sharon R, McMaster P R, Kask A M, Owens J D, Paul W E
J Immunol. 1975 May;114(5):1585-9.
The 2,4-dinitrophenyl-lysyl derivative of Ficoll (DNP-Lys-Ficoll) was prepared and examined for immunogenicity. This antigen elicited large numbers of DNP-specific plaque-forming cells (PFC) of the IgM and IgG2 class in the spleens of C57BL/6 mice. Similar responses were observed in congenitally athymic (nu/nu) mice and in their littermates indicating that DNP-Lys-Ficoll is a T-independent antigen. The responses of nu/nu mice included a large number of IgG2 DNP-specific PFC, indicating that IgG responses can be initiated in the absence of mature thymus-dependent (T) lymphocytes. Cell transfer studies confirmed the T independence of the response and indicated that priming with DNP-Lys-Ficoll induces only a very meager degree of memory. Because they can be obtained in large quantities and in relatively pure form, DNP-Lys-Ficoll and other hapten conjugates of Ficoll should prove most valuable in the delineation of the mode of activation of precursors of antibody-secreting cells by T-independent antigens.
制备了聚蔗糖的2,4 - 二硝基苯基 - 赖氨酰衍生物（DNP - Lys - 聚蔗糖）并检测其免疫原性。该抗原在C57BL/6小鼠脾脏中引发了大量IgM和IgG2类的DNP特异性空斑形成细胞（PFC）。在先天性无胸腺（nu/nu）小鼠及其同窝仔鼠中观察到类似反应，表明DNP - Lys - 聚蔗糖是一种非T细胞依赖性抗原。nu/nu小鼠的反应包括大量IgG2 DNP特异性PFC，表明在没有成熟的胸腺依赖性（T）淋巴细胞的情况下也可启动IgG反应。细胞转移研究证实了该反应的非T细胞依赖性，并表明用DNP - Lys - 聚蔗糖致敏仅诱导非常微弱程度的记忆。由于DNP - Lys - 聚蔗糖和聚蔗糖的其他半抗原缀合物能够大量且相对纯地获得，它们在描绘非T细胞依赖性抗原激活抗体分泌细胞前体的模式方面应具有很大价值。
